FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152572

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152572
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5944
Total Bases861.5 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC162627.355316285329746No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC104317.547106325706597No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG79113.307537012113055No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC5759.673620457604306No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC3225.417227456258412No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG1342.2543741588156125No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG711.1944818304172276No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA430.7234185733512786No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT380.639300134589502No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT320.5383580080753702No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT250.4205921938088829No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA240.4037685060565276No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA210.3532974427994616No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTGG200.3364737550471063No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTGGCTAATGTAGAACCATGT190.319650067294751No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA160.2691790040376851No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA160.2691790040376851No Hit
AAATACCGTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT160.2691790040376851No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT150.2523553162853297No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA140.23553162853297444No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT140.23553162853297444No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA140.23553162853297444No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA140.23553162853297444No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA140.23553162853297444No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA120.2018842530282638No Hit
AAATACGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT110.18506056527590847No Hit
GGCACTAGAATGGACCTCTTCGTCATCACTAAATTATTTCAATTAAATAC100.16823687752355315No Hit
AAATACCGTTCAGAGCTGCCCCCAAGAGCTTGCAAATTGCTGCTGGAACC90.15141318977119786No Hit
AAATACCGTTAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT90.15141318977119786No Hit
GGCACTAGAATGGACCTCTTCCCCATCACTAAATTATTTCAATTAAATAC80.13458950201884254No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAAATAAATAC70.11776581426648722No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA70.11776581426648722No Hit
AAATACCGTTCAGAGCTGCCACCAAGGGCTTGCAAATTGCTGCTGGAACC70.11776581426648722No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA60.1009421265141319No Hit
GGCACTAGAATGGCCTCTTCCTCATCACTAAATTATTTCAATTAAATACA60.1009421265141319No Hit
GGCACTAGAATGGACCTCTTCCTCATAACTAAATTATTTCAATTAAATAC60.1009421265141319No Hit
GGCACTAGAATGGACCCTTCCTCATCACTAAATTATTTCAATTAAATACA60.1009421265141319No Hit
AAATACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT60.1009421265141319No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA60.1009421265141319No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATCATTTCAATTAAATAC60.1009421265141319No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGATTGCAAATTGCTGCTGGAACC60.1009421265141319No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA60.1009421265141319No Hit
AAATACCGTTCAGGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT60.1009421265141319No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)