FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152581

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152581
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4809
Total Bases697.2 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCT184738.40715325431482No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTTGGACAGACCAAGTCTC96420.045747556664587No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG91318.985236015803704No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTTTGGACAGACCAAGTCTC2515.219380328550635No Hit
ACTTAAAAACAAGGCAGTGCTGAACGGTATTTTTGGACAGACCAAGTCTC611.268454980245373No Hit
ACTTAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC410.8525681014763984No Hit
ACTTAAAAACAAGGCAGTGCTGTGCCCTCCCAGGTACCCCAAGCTGGCAG370.7693907257226035No Hit
ACTTAAAAACAAGGCAGTGCTATGCCCTCCCAGGTACCCCAAGCTGGCAG210.43668122270742354No Hit
ACTTAAAAACAAGGCAGTGCTATCAACGAAGAGGTCCATTCTAGTGCCTT190.3950925348305261No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTGGACAGACCAAGTCTCT190.3950925348305261No Hit
ACTTAAAAACAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC170.35350384695362863No Hit
ACTTAAAACAAGGCAGCTCTGAACGGTATTTTTGGACAGACCAAGTCTCT130.27032647119983366No Hit
ACTTAAAAACAAGGCAGCTCTGAACGTATTTTTGGACAGACCAAGTCTCT90.18714909544603867No Hit
ACTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC90.18714909544603867No Hit
ACTTAAAAAAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC80.16635475150758994No Hit
ACTTAAAAACAAGGCAGTGTTAATGCCTAATGGTGCTACAGTTTCTGCCT70.1455604075691412No Hit
ACTTAAAAACAAGGCAGTTCTAATGCCTAATGGTGCTACAGTTTCTGCCT70.1455604075691412No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAGTGGTGCTACAGTTTCTGCCT70.1455604075691412No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCC70.1455604075691412No Hit
TGTGTGTGTGTTTCTGTGGGTAAGCAGTGGTATCAACGAAGAGGTCCATT70.1455604075691412Clontech SMARTer II A Oligonucleotide (95% over 22bp)
ACTTAAAAACAAGGCAGCTCTGAACGGTTTTTTGGACAGACCAAGTCTCT60.12476606363069245No Hit
ACTTAAAAACAAGGCAGCTCTGAAGGTATTTTTGGACAGACCAAGTCTCT60.12476606363069245No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTTGGACAGACCAAGTCTCT60.12476606363069245No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTTGGACAGCCCAAGTCTC60.12476606363069245No Hit
ACTTAAAAACAAGGCAGCTCTGACGGTATTTTTGGACAGACCAAGTCTCT60.12476606363069245No Hit
ATTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC60.12476606363069245No Hit
ACTTAAAAACAAGGCAGTGCTAATGACTAATGGTGCTACAGTTTCTGCCT50.1039717196922437No Hit
ACTTAAAAACAAGGCAGTGCTGATGCCTAATGGTGCTACAGTTTCTGCCT50.1039717196922437No Hit
ACTTAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTCT50.1039717196922437No Hit
TGTGTGTGTGTTTCTGTGGCAGCTCTGAACGGTATTTTTGGACAGACCAA50.1039717196922437No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGTCT50.1039717196922437No Hit
ACTTAAAAACAAGGCAGTGGTATCAACGAAGAGGTCCATTCTAGTGCCTT50.1039717196922437No Hit
ACTTAAAAACAAGGCAGCGCTAATGCCTAATGGTGCTACAGTTTCTGCCT50.1039717196922437No Hit
ACTAAAAACAAGGCAGCTCTGAACGGTATTTTTGGACAGACCAAGTCTCT50.1039717196922437No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACGGTTTCTGCCT50.1039717196922437No Hit
ACTTAAAAACAAGGCACTCTGAACGGTATTTTTGGACAGACCAAGTCTCT50.1039717196922437No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)