FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152595

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152595
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5730
Total Bases830.7 kbp
Sequences flagged as poor quality0
Sequence length144-145
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCT267146.61431064572426No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTTGGACAGACCAAGTCTC122521.37870855148342No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG5259.162303664921465No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTTTGGACAGACCAAGTCTC3065.340314136125655No Hit
ACTTAAAAACAAGGCAGTGCTGAACGGTATTTTTGGACAGACCAAGTCTC681.1867364746945899No Hit
ACTTAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC651.1343804537521813No Hit
ACTTAAAAACAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC320.5584642233856894No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTGGACAGACCAAGTCTCT280.48865619546247824No Hit
ACTTAAAACAAGGCAGCTCTGAACGGTATTTTTGGACAGACCAAGTCTCT260.45375218150087265No Hit
ACTTAAAAACAAGGCAGTGCTGTGCCCTCCCAGGTACCCCAAGCTGGCAG170.29668411867364747No Hit
ACTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC160.2792321116928447No Hit
ACTTAAAAACAAGGCAGTGCTATCAACGAAGAGGTCCATTCTAGTGCCTT150.2617801047120419No Hit
ACTTAAAAACAAGGCAGCTCTGAACGTATTTTTGGACAGACCAAGTCTCT140.24432809773123912No Hit
ATTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC130.22687609075043633No Hit
ACTTAAAAAAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC120.20942408376963353No Hit
ACTTAAAAACAAGGCAGTGCTATGCCCTCCCAGGTACCCCAAGCTGGCAG120.20942408376963353No Hit
ACTTAAAAACAAGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC100.17452006980802792No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTTGGACAGACCAAGTCTCT100.17452006980802792No Hit
ACTTAAAAACAAGGCAGCTCTGACGGTATTTTTGGACAGACCAAGTCTCT100.17452006980802792No Hit
ACTTAAAAACAAGGCAGTCTGAACGGTATTTTTGGACAGACCAAGTCTCT100.17452006980802792No Hit
ACTTAAAAACAAGGCGGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCT100.17452006980802792No Hit
ACTTAAAAACAAGGCAGTGCTAATGACTAATGGTGCTACAGTTTCTGCCT90.15706806282722513No Hit
ACTTAAAAACAAGGCAGCTCTGAAGGTATTTTTGGACAGACCAAGTCTCT90.15706806282722513No Hit
ACTTAAAAACAAGGCAGCGCTAATGCCTAATGGTGCTACAGTTTCTGCCT90.15706806282722513No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTACCT80.13961605584642234No Hit
ACTAAAAACAAGGCAGCTCTGAACGGTATTTTTGGACAGACCAAGTCTCT80.13961605584642234No Hit
CCTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCT80.13961605584642234No Hit
CTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC70.12216404886561956No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTCTCTGCCT70.12216404886561956No Hit
ACTTAAAAACAAGGCAGTGCTAGTGCCTAATGGTGCTACAGTTTCTGCCT70.12216404886561956No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGTCT70.12216404886561956No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCCGCCT70.12216404886561956No Hit
TGTGTGTGTGTTTCTGTGGCAGCTCTGAACGGTATTTTTGGACAGACCAA60.10471204188481677No Hit
TGTGTGTGTGTTTCTGTGGGTAAGCAGTGGTATCAACGAAGAGGTCCATT60.10471204188481677Clontech SMARTer II A Oligonucleotide (95% over 22bp)
ACTTAAAAACAAGGCAGTTCTAATGCCTAATGGTGCTACAGTTTCTGCCT60.10471204188481677No Hit
ACTTAAAAACAAGGCAGTGCTAATGTCTAATGGTGCTACAGTTTCTGCCT60.10471204188481677No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCC60.10471204188481677No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)