FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152596

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152596
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5730
Total Bases830.7 kbp
Sequences flagged as poor quality0
Sequence length141-145
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC132923.19371727748691No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG119120.785340314136125No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC112219.581151832460733No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC4307.504363001745201No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG3015.2530541012216405No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG641.1169284467713787No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT390.6806282722513088No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT340.5933682373472949No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT270.4712041884816754No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTGGCTAATGTAGAACCATGT260.45375218150087265No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC200.34904013961605584No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTGG160.2792321116928447No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT160.2792321116928447No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT150.2617801047120419No Hit
AAATACGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT140.24432809773123912No Hit
AAATACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT140.24432809773123912No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT140.24432809773123912No Hit
AAATACCGTTAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT130.22687609075043633No Hit
AAATACCGTTCAGACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT110.19197207678883071No Hit
AATACCGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT100.17452006980802792No Hit
AAATACCGTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT100.17452006980802792No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGATTGCAAATTGCTGCTGGAACC100.17452006980802792No Hit
AAATACCGTTCAAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT90.15706806282722513No Hit
AAATACCTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT90.15706806282722513No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAGTGGCTAATGTAGAACCATGT90.15706806282722513No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA80.13961605584642234No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA80.13961605584642234No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGATGCTGGAACC80.13961605584642234No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGATGCTGGAACC80.13961605584642234No Hit
AAATACCGTTCAAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT80.13961605584642234No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCTTACCCACAGAAACA70.12216404886561956Clontech SMARTer II A Oligonucleotide (95% over 22bp)
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT70.12216404886561956No Hit
AAATACCGTTCGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT70.12216404886561956No Hit
AAATACCGTTCGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT70.12216404886561956No Hit
AAATACCGTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT70.12216404886561956No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGACCC70.12216404886561956No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTAGAACC60.10471204188481677No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGACCC60.10471204188481677No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACAGGCTAATGTAGAA60.10471204188481677No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)