FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152620

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152620
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4107
Total Bases595.3 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC86521.061602142683224No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC72517.652787923058195No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG55813.586559532505479No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC50112.198685171658145No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC4099.958607255904553No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG1654.017531044558072No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG410.9982955928901875No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT180.4382761139517896No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT180.4382761139517896No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA160.38957876795714635No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT160.38957876795714635No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA110.2678354029705381No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTGG110.2678354029705381No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT110.2678354029705381No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA100.24348672997321646No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTGGCTAATGTAGAACCATGT100.24348672997321646No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA100.24348672997321646No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA90.2191380569758948No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA90.2191380569758948No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA80.19478938397857318No Hit
AAATACCGTTAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT70.17044071098125152No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTAAATTATTTCAATTAAATAC70.17044071098125152No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA70.17044071098125152No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA60.1460920379839299No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA60.1460920379839299No Hit
GGCACTAGAATGGACCCTTCCTCATCACTAAATTATTTCAATTAAATACA60.1460920379839299No Hit
AAATACCGTTCAGAACTGCCTTGTTTTAAGTGGCTAATGTAGAACCATGT60.1460920379839299No Hit
AAATACCGTTCAGAGTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT60.1460920379839299No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT60.1460920379839299No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA50.12174336498660823No Hit
GGCACTAGAATGGACCTCTTCCTCATCACCACATTATTTCAATTAAATAC50.12174336498660823No Hit
AAATACGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT50.12174336498660823No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA50.12174336498660823No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)