FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152632

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152632
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7287
Total Bases1 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC183325.15438452037876No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG109515.026759983532317No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC94212.927130506381227No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC87111.952792644435297No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC5627.712364484698779No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG2373.2523672293124744No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG1121.536983669548511No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT440.603815013036915No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT370.5077535336901331No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTGG360.49403046521202143No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT310.4254151228214629No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA290.3979689858652395No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA250.34307671195279266No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTGGCTAATGTAGAACCATGT240.32935364347468093No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT210.2881844380403458No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA200.27446136956223416No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA190.26073830108412244No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA180.24701523260601072No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA150.20584602717167558No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA140.19212295869356388No Hit
AAATACGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT130.1783998902154522No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA130.1783998902154522No Hit
AAATACCGTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT120.16467682173734047No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA110.15095375325922875No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA110.15095375325922875No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT110.15095375325922875No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA110.15095375325922875No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA110.15095375325922875No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA100.13723068478111708No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA100.13723068478111708No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTAAATTATTTCAATTAAATAC100.13723068478111708No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA100.13723068478111708No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCTTACCCACAGAAACA90.12350761630300536Clontech SMARTer II A Oligonucleotide (95% over 22bp)
GGCACTAGAATGGACCTCTTCGTCATCACTAAATTATTTCAATTAAATAC90.12350761630300536No Hit
AAATACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT90.12350761630300536No Hit
AAATCCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT80.10978454782489364No Hit
AAATACCGTTAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT80.10978454782489364No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACAGGCTAATGTAGAA80.10978454782489364No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA80.10978454782489364No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTAAATTAAATAC80.10978454782489364No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)