FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152643

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152643
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5127
Total Bases743.3 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG309060.269163253364546No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTTGGACAGACCAAGTCTC86216.81295104349522No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTTTGGACAGACCAAGTCTC2134.154476301930954No Hit
ACTTAAAAACAAGGCAGTGCTGAACGGTATTTTTGGACAGACCAAGTCTC721.4043300175541251No Hit
ACTTAAAAACAAGGCAGTGCTGTGCCCTCCCAGGTACCCCAAGCTGGCAG591.150770431051297No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCT510.994733762434172No Hit
ACTTAAAAACAAGGCAGTGCTATGCCCTCCCAGGTACCCCAAGCTGGCAG400.7801833430856251No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTGGACAGACCAAGTCTCT240.4681100058513751No Hit
TGTGTGTGTGTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC230.44860542227423444No Hit
ACTTAAAAACAAGGCAGTGCTATCAACGAAGAGGTCCATTCTAGTGCCTT220.42910083869709387No Hit
ACTTAAAACAAGGCAGCTCTGAACGGTATTTTTGGACAGACCAAGTCTCT220.42910083869709387No Hit
TGTGTGTGTGTTTTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC150.29256875365710944No Hit
TGTGTGTGTGTTTCTGTGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC150.29256875365710944No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGGCAGAAGGGTAAAG140.2730641700799688No Hit
TGTGTGTGTGTTTCTGTGGGTTTCCTTAAGGTTTGGACAGAAGGGTAAAG130.25355958650282817No Hit
TGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGCT130.25355958650282817No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACGGAAGGGTAAAG130.25355958650282817No Hit
ACTTAAAAACAAGGCAGCTCTAACGGTATTTTTGGACAGACCAAGTCTCT110.21455041934854693No Hit
ACTTAAAAACAAGGCAGCTCTGAACGTATTTTTGGACAGACCAAGTCTCT100.19504583577140627No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAGG90.17554125219426564No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAGAG90.17554125219426564No Hit
GTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC90.17554125219426564No Hit
TGTTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC90.17554125219426564No Hit
ACTTAAAACAAGGCAGTTCTGAACGGTATTTTTGGACAGACCAAGTCTCT90.17554125219426564No Hit
TGTGTGTGTGTTTCTGTGGGTAAGCAGTGGTATCAACGAAGAGGTCCATT90.17554125219426564Clontech SMARTer II A Oligonucleotide (95% over 22bp)
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGGAGGGTAAAG80.15603666861712503No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTGAAG80.15603666861712503No Hit
TGTGGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC80.15603666861712503No Hit
TGTGTTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC80.15603666861712503No Hit
ACTTAAAAACAAGGCAGTCTGAACGGTATTTTTGGACAGACCAAGTCTCT70.1365320850399844No Hit
ACTTAAAAACAAGGCAGCTCGAACGGTATTTTTGGACAGACCAAGTCTCT70.1365320850399844No Hit
TGTGTGTGTGTTTCTGTGGGCTTCTTTAAGGTTTGGACAGAAGGGTAAAG70.1365320850399844No Hit
ACTTAAAAACAAGGCAGTGGTATCAACGAAGAGGTCCATTCTAGTGCCTT70.1365320850399844No Hit
TGGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC70.1365320850399844No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAGGGTTTGGACAGAAGGGTAAAG70.1365320850399844No Hit
TTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC60.11702750146284377No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAA60.11702750146284377No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTCTAAGGTTTGGACAGAAGGGTAAAG60.11702750146284377No Hit
TGTGTGTGGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC60.11702750146284377No Hit
ACTAAAAACAAGGCAGCTCTGAACGGTATTTTTGGACAGACCAAGTCTCT60.11702750146284377No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)