FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152656

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152656
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3381
Total Bases490.1 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC80623.8391008577344No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG76422.596864832889676No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC47113.930789707187222No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC3379.967465246968352No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG2828.34072759538598No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG300.8873114463176575No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTGGCTAATGTAGAACCATGT260.7690032534753032No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA200.5915409642117717No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT200.5915409642117717No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA190.5619639160011831No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA120.354924578527063No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA110.3253475303164744No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT100.29577048210588586No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT90.26619343389529726No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCCTTGTTTTTAAGTGG70.2070393374741201No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT70.2070393374741201No Hit
AAATACGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT60.1774622892635315No Hit
AATACCGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT60.1774622892635315No Hit
AAATACCGTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT60.1774622892635315No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA60.1774622892635315No Hit
AAATACCGTTCAGAGTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT60.1774622892635315No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT60.1774622892635315No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA50.14788524105294293No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA50.14788524105294293No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA50.14788524105294293No Hit
GGCACTAGAATGGACCTCTTCGTCATCACTACATTATTTCAATTAAATAC50.14788524105294293No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT40.11830819284235432No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACACTATTTCAATTAAATAC40.11830819284235432No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACGTTATTTCAATTAAATAC40.11830819284235432No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT40.11830819284235432No Hit
AAAACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT40.11830819284235432No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGATGCTGGAACC40.11830819284235432No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA40.11830819284235432No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA40.11830819284235432No Hit
GGCACTAGAATGGACCTCTTCCCCATCACTACATTATTTCAATTAAATAC40.11830819284235432No Hit
AAATACCGTTAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT40.11830819284235432No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)