FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152658

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152658
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9054
Total Bases1.3 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC233025.734481996907444No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC145616.081290037552463No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG128214.159487519328472No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC100611.11111111111111No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC6086.715263971725205No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG4815.312569030262868No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT640.7068698917605478No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG540.5964214711729622No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT420.46388336646785955No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA380.41970399823282534No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT280.30925557764523964No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTGGCTAATGTAGAACCATGT260.28716589352772254No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA230.2540313673514469No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA230.2540313673514469No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA210.23194168323392977No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA210.23194168323392977No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA170.1877623149988955No Hit
AAATACCGTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT170.1877623149988955No Hit
AAATACGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT160.17671747294013695No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA150.16567263088137837No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA150.16567263088137837No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT150.16567263088137837No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGAAAGCCAATGGGAAA150.16567263088137837No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTGG140.15462778882261982No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAGTGGCTAATGTAGAACCATGT140.15462778882261982No Hit
AATACCGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT130.14358294676386127No Hit
AAATACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT130.14358294676386127No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA130.14358294676386127No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA130.14358294676386127No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA110.12149326264634416No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA110.12149326264634416No Hit
AAATACCGTTCAGAACTGCCTTGTTTTAAGTGGCTAATGTAGAACCATGT100.11044842058758561No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA100.11044842058758561No Hit
ACCTTAAAGAAACCCACAGAAACACACACACAGGCTAATGTAGAACCATG100.11044842058758561No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA100.11044842058758561No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT100.11044842058758561No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA100.11044842058758561No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)