FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152661

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152661
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8184
Total Bases1.1 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG459656.158357771260995No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTTGGACAGACCAAGTCTC113013.807429130009774No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCT103312.62218963831867No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTTTGGACAGACCAAGTCTC2583.1524926686217007No Hit
ACTTAAAAACAAGGCAGTGCTGAACGGTATTTTTGGACAGACCAAGTCTC610.7453567937438905No Hit
ACTTAAAAACAAGGCAGTGCTATCAACGAAGAGGTCCATTCTAGTGCCTT500.6109481915933529No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTGGACAGACCAAGTCTCT400.4887585532746823No Hit
TGTGTGTGTGTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC300.36656891495601174No Hit
ACTTAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC230.28103616813294235No Hit
ACTTAAAACAAGGCAGCTCTGAACGGTATTTTTGGACAGACCAAGTCTCT200.24437927663734116No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAGGGTTTGGACAGAAGGGTAAAG170.20772238514173996No Hit
ACTTAAAAACAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC140.1710654936461388No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAGG120.1466275659824047No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAGGGGTAAAG120.1466275659824047No Hit
TGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGCT120.1466275659824047No Hit
TGTGTGTGTGTTTCTGTGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC120.1466275659824047No Hit
ACTTAAAAACAAGGCAGCTCTGAACGTATTTTTGGACAGACCAAGTCTCT110.13440860215053765No Hit
TGTGTGTGTGTTTCTGTGGGTTTCCTTAAGGTTTGGACAGAAGGGTAAAG110.13440860215053765No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTCGGACAGAAGGGTAAAG100.12218963831867058No Hit
TGTGTGTGTGTTTCTGTGGGTCTCTTTAAGGTTTGGACAGAAGGGTAAAG100.12218963831867058No Hit
TGTTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC100.12218963831867058No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGGCAGAAGGGTAAAG100.12218963831867058No Hit
TGGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC100.12218963831867058No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACGGAAGGGTAAAG90.10997067448680353No Hit
ACTTAAAACAAGGCAGTTCTGAACGGTATTTTTGGACAGACCAAGTCTCT90.10997067448680353No Hit
ACTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC90.10997067448680353No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)