FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152670

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152670
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8480
Total Bases1.2 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC266131.379716981132077No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC144817.075471698113205No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG110313.007075471698112No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC90410.660377358490566No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG2723.207547169811321No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC2192.582547169811321No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG830.9787735849056604No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA380.4481132075471698No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTGG360.42452830188679247No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA330.3891509433962264No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT320.37735849056603776No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA320.37735849056603776No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA290.3419811320754717No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA290.3419811320754717No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA280.33018867924528306No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA240.2830188679245283No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA190.2240566037735849No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA190.2240566037735849No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA190.2240566037735849No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTGGCTAATGTAGAACCATGT180.21226415094339623No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA180.21226415094339623No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA170.20047169811320756No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT170.20047169811320756No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA170.20047169811320756No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA160.18867924528301888No Hit
GGCACTAGAATGGACCTCTTCGTCATCACTACATTATTTCAATTAAATAC150.1768867924528302No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCTTACCCACAGAAACA130.15330188679245285Clontech SMARTer II A Oligonucleotide (95% over 22bp)
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA130.15330188679245285No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA130.15330188679245285No Hit
ACCTTAAAGAAACCCACAGAAACACACACACAGGCTAATGTAGAACCATG120.14150943396226415No Hit
AAATACGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT110.12971698113207547No Hit
GGCACTAGAATGGACCTCTTCCTCACCACTACATTATTTCAATTAAATAC110.12971698113207547No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCCTTGTTTTTAAGTGG90.10613207547169812No Hit
AAATACCGTTCAGACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT90.10613207547169812No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTAAATTAAATAC90.10613207547169812No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)