FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152671

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152671
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4258
Total Bases617.3 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCT143933.79520901831846No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG104824.61249412869892No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTTGGACAGACCAAGTCTC77318.154062940347583No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTTTGGACAGACCAAGTCTC1794.20385157350869No Hit
ACTTAAAAACAAGGCAGTGCTGAACGGTATTTTTGGACAGACCAAGTCTC922.1606387975575387No Hit
ACTTAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC400.9394081728511038No Hit
TGTGTGTGTGTTTCTGTGGGTCCAGTTTGCTTGGCGAAGAGGTCCATTCT280.6575857209957726No Hit
TGTGTGTGTGTTTCTGTGGGTCCAGTTTGCTTGGCGTAGAGGTCCATTCT210.4931892907468295No Hit
ACTTAAAACAAGGCAGCTCTGAACGGTATTTTTGGACAGACCAAGTCTCT160.37576326914044156No Hit
ACTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC140.3287928604978863No Hit
ACTTAAAAACAAGGCAGCGCTAATGCCTAATGGTGCTACAGTTTCTGCCT110.25833724753405357No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTGGACAGACCAAGTCTCT110.25833724753405357No Hit
ACTTAAAAACAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC100.23485204321277595No Hit
ACTTAAAAACAAGGCAGCTCTGAACGTATTTTTGGACAGACCAAGTCTCT100.23485204321277595No Hit
ACTTAAAAAAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC100.23485204321277595No Hit
ACTTAAAAACAAGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC90.21136683889149835No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGCGCTACAGTTTCTGCCT90.21136683889149835No Hit
ACTTAAAAACAAGGCAGTGCTAATGACTAATGGTGCTACAGTTTCTGCCT70.16439643024894315No Hit
TGTGTGTGTGTTTCTGTGGGCCAGTTTGCTTGGCGTAGAGGTCCATTCTA70.16439643024894315No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGTCT70.16439643024894315No Hit
TGTGTGTGTGTTTCTGTGGGCCCAGTTTGCTTGGCGAAGAGGTCCATTCT70.16439643024894315No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAGG60.14091122592766556No Hit
TGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGCT60.14091122592766556No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCCGCCT60.14091122592766556No Hit
ACTAAAAACAAGGCAGCTCTGAACGGTATTTTTGGACAGACCAAGTCTCT60.14091122592766556No Hit
ATTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC60.14091122592766556No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCC60.14091122592766556No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAGGGTTTGGACAGAAGGGTAAAG60.14091122592766556No Hit
CCTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCT50.11742602160638797No Hit
ACTTAAAAACAAGGCAGCTCTAACGGTATTTTTGGACAGACCAAGTCTCT50.11742602160638797No Hit
ACTTAAAAACAAGGCAGTGCTATCAACGAAGAGGTCCATTCTAGTGCCTT50.11742602160638797No Hit
TGTGTGTGTGTTTTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC50.11742602160638797No Hit
ACTTAAAAACAAGGCACTCTGAACGGTATTTTTGGACAGACCAAGTCTCT50.11742602160638797No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)