FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152684

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152684
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5158
Total Bases747.5 kbp
Sequences flagged as poor quality0
Sequence length141-145
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC152929.64327258627375No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC96918.786351298953083No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG75214.579294300116324No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC4528.76308646762311No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG1883.644823575029081No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG470.9112058937572702No Hit
GGCACTAGAATGGACCTCTTCCTCATCACCAAATTATTTCAATTAAATAC460.8918185343156262No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT450.8724311748739823No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA420.8142690965490499No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA300.5816207832493214No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA230.4459092671578131No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA200.38774718883288095No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTGGCTAATGTAGAACCATGT180.34897246994959286No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA170.3295851105079488No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA150.2908103916246607No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA150.2908103916246607No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA150.2908103916246607No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA150.2908103916246607No Hit
GGCACTAGAATGGACCTCTTCGTCATCACTAAATTATTTCAATTAAATAC140.27142303218301667No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT140.27142303218301667No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA130.2520356727413726No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT130.2520356727413726No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA120.23264831329972857No Hit
AAATACCGTTCAGACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT100.19387359441644048No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT100.19387359441644048No Hit
AAATACGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT90.17448623497479643No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA90.17448623497479643No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA80.15509887553315238No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA80.15509887553315238No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA80.15509887553315238No Hit
GGCACTAGAATGGACCTCTTCCTCACCACTAAATTATTTCAATTAAATAC80.15509887553315238No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAGTGGCTAATGTAGAACCATGT80.15509887553315238No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA70.13571151609150833No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTGG70.13571151609150833No Hit
GGCACTAGAATGGCCTCTTCCTCATCACTAAATTATTTCAATTAAATACA70.13571151609150833No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTAAATTATTTCAATTAAATAC70.13571151609150833No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA70.13571151609150833No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA70.13571151609150833No Hit
GGCACTAGAATGGACCTCTTACTCATCACTAAATTATTTCAATTAAATAC70.13571151609150833No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA60.11632415664986429No Hit
GGCACTAGAATGGACCTCTCCCTCATCACTAAATTATTTCAATTAAATAC60.11632415664986429No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA60.11632415664986429No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTAAATTAAATAC60.11632415664986429No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)