FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152688

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152688
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6221
Total Bases901.8 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC189630.477415206558433No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG101016.235331940202542No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC79412.763221347050314No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC5969.58045330332744No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG2814.5169586883137764No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGAAACC2413.8739752451374376No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG701.125221025558592No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT460.7394309596527889No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT330.530461340620479No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA280.45008841022343676No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT260.4179392380646198No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT230.36971547982639447No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA180.28934254942935217No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA170.27326796334994374No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTGGCTAATGTAGAACCATGT160.2571933772705353No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA130.2089696190323099No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA110.176820446873493No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA110.176820446873493No Hit
GGCACTAGAATGGACCTCTTCCTCATCGCTACATTATTTCAATTAAATAC110.176820446873493No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTGG100.16074586079408454No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA100.16074586079408454No Hit
AAATACGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT100.16074586079408454No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT90.14467127471467608No Hit
GGCACTAGAATGGACCCCTTCCTCATCACTACATTATTTCAATTAAATAC80.12859668863526766No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGAAACC80.12859668863526766No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA80.12859668863526766No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA70.11252210255585919No Hit
AAATACCGTTCAGACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT70.11252210255585919No Hit
AAATACCGTTAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT70.11252210255585919No Hit
AAATACCGTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT70.11252210255585919No Hit
GGCACTAGAATGGCCTCTTCCTCATCACTACATTATTTCAATTAAATACA70.11252210255585919No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA70.11252210255585919No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)