FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152689

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152689
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6304
Total Bases914 kbp
Sequences flagged as poor quality0
Sequence length141-145
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCT184829.314720812182742No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG177728.18845177664975No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTTGGACAGACCAAGTCTC116218.43274111675127No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTTTGGACAGACCAAGTCTC4617.312817258883249No Hit
ACTTAAAAACAAGGCAGTGCTGTGCCCTCCCAGGTACCCCAAGCTGGCAG981.5545685279187818No Hit
ACTTAAAAACAAGGCAGTGCTGAACGGTATTTTTGGACAGACCAAGTCTC480.7614213197969544No Hit
ACTTAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC400.6345177664974619No Hit
ACTTAAAAACAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC260.4124365482233503No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTGGACAGACCAAGTCTCT230.36484771573604063No Hit
ACTTAAAACAAGGCAGCTCTGAACGGTATTTTTGGACAGACCAAGTCTCT200.31725888324873097No Hit
ACTTAAAAACAAGGCAGTGCTATGCCCTCCCAGGTACCCCAAGCTGGCAG200.31725888324873097No Hit
ACTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC180.28553299492385786No Hit
ACTTAAAAACAAGGCAGTCTGAACGGTATTTTTGGACAGACCAAGTCTCT160.25380710659898476No Hit
ACTTAAAAACAAGGCAGTTCTAATGCCTAATGGTGCTACAGTTTCTGCCT130.20621827411167515No Hit
TGTGTGTGTGTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC120.1903553299492386No Hit
ACTTAAAAAAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC100.15862944162436549No Hit
ACTTAAAAACAAGGCAGCTCTAACGGTATTTTTGGACAGACCAAGTCTCT100.15862944162436549No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTTGGACAGACCAAGTCTCT90.14276649746192893No Hit
ACTTAAAAACAAGGCAGCTCTGACGGTATTTTTGGACAGACCAAGTCTCT90.14276649746192893No Hit
ACTTAAAAACAAGGCAGTGCTATCAACGAAGAGGTCCATTCTAGTGCCTT90.14276649746192893No Hit
ACTTAAAAACAAGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC80.12690355329949238No Hit
TGTGTGTGTGTTTCTGTGGCAGCTCTGAACGGTATTTTTGGACAGACCAA80.12690355329949238No Hit
ACTTAAAAACAAGGCAGCTCTGAACGTATTTTTGGACAGACCAAGTCTCT80.12690355329949238No Hit
ACTTAAAACAAGGCAGTTCTGAACGGTATTTTTGGACAGACCAAGTCTCT80.12690355329949238No Hit
ACTTAAAAACAAGGCGGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCT80.12690355329949238No Hit
ACTTAAAAACAAGGCAGCGCTAATGCCTAATGGTGCTACAGTTTCTGCCT80.12690355329949238No Hit
ACTTAAAAACAAGGCAGTGCTGATGCCTAATGGTGCTACAGTTTCTGCCT70.11104060913705585No Hit
TGGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC70.11104060913705585No Hit
ACTTAAAAACAAGGCACTCTGAACGGTATTTTTGGACAGACCAAGTCTCT70.11104060913705585No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)