FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152702

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152702
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8063
Total Bases1.1 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC176721.914920004960933No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC161119.980156269378643No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG121815.106039935507876No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC99312.315515316879573No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC3974.923725660424159No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG2843.5222621852908342No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG891.1038075158129728No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA320.3968746124271363No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA260.3224606225970482No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTGG260.3224606225970482No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT260.3224606225970482No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA240.29765595932035227No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT240.29765595932035227No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTGGCTAATGTAGAACCATGT230.28525362768200424No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA230.28525362768200424No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA210.2604489644053082No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA200.24804663276696018No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA190.2356443011286122No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA190.2356443011286122No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA160.19843730621356814No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA160.19843730621356814No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA150.18603497457522014No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA130.1612303112985241No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA130.1612303112985241No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA120.14882797966017614No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA120.14882797966017614No Hit
AAATACGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT110.1364256480218281No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA110.1364256480218281No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA110.1364256480218281No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCCTTGTTTTTAAGTGG100.12402331638348009No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCTTACCCACAGAAACA100.12402331638348009Clontech SMARTer II A Oligonucleotide (95% over 22bp)
GGCACTAGAATGGACCTCTTCGTCATCACTAAATTATTTCAATTAAATAC100.12402331638348009No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA100.12402331638348009No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT100.12402331638348009No Hit
AAATACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT100.12402331638348009No Hit
AAATACCGTTCAGAGTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT100.12402331638348009No Hit
AAATACCGTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT100.12402331638348009No Hit
AAAACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT90.11162098474513209No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)