FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152731

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152731
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4544
Total Bases658.8 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCT177939.15052816901409No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTTGGACAGACCAAGTCTC88619.49823943661972No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG86218.970070422535212No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTTTGGACAGACCAAGTCTC1884.137323943661972No Hit
ACTTAAAAACAAGGCAGTGCTGAACGGTATTTTTGGACAGACCAAGTCTC591.2984154929577465No Hit
ACTTAAAAACAAGGCAGTGCTGTGCCCTCCCAGGTACCCCAAGCTGGCAG581.2764084507042255No Hit
ACTTAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC531.1663732394366197No Hit
ACTTAAAAACAAGGCAGTGCTATGCCCTCCCAGGTACCCCAAGCTGGCAG290.6382042253521127No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTGGACAGACCAAGTCTCT250.5501760563380281No Hit
ACTTAAAAACAAGGCAGTGCTATCAACGAAGAGGTCCATTCTAGTGCCTT230.506161971830986No Hit
ACTTAAAAACAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC170.37411971830985913No Hit
ACTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC140.30809859154929575No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCCGCCT130.2860915492957747No Hit
ACTTAAAACAAGGCAGCTCTGAACGGTATTTTTGGACAGACCAAGTCTCT110.24207746478873238No Hit
ACTTAAAAACAAGGCAGTGGTATCAACGAAGAGGTCCATTCTAGTGCCTT80.17605633802816903No Hit
ATTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC80.17605633802816903No Hit
TGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGCT70.15404929577464788No Hit
ACTTAAAAACAAGGCAGCTCTGACGGTATTTTTGGACAGACCAAGTCTCT70.15404929577464788No Hit
ACTTAAAAACAAGGCGGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCT70.15404929577464788No Hit
ACTTAAAAACAAGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC60.13204225352112675No Hit
ACTTAAAAACAAGGCAGCTTGAACGGTATTTTTGGACAGACCAAGTCTCT60.13204225352112675No Hit
ACTTAAAAACAAGGCAGTCTGAACGGTATTTTTGGACAGACCAAGTCTCT60.13204225352112675No Hit
ACTTAAAAACAAGGCAGCTCGAACGGTATTTTTGGACAGACCAAGTCTCT60.13204225352112675No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGTTACAGTTTCTGCCT50.11003521126760564No Hit
CTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC50.11003521126760564No Hit
CCTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCT50.11003521126760564No Hit
ACTTAAAAACAAGGCAGTGCTGATGCCTAATGGTGCTACAGTTTCTGCCT50.11003521126760564No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCCAATGGTGCTACAGTTTCTGCCT50.11003521126760564No Hit
ACTTAAAAACAAGGCAGTGCTAGTGCCTAATGGTGCTACAGTTTCTGCCT50.11003521126760564No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGCGCTACAGTTTCTGCCT50.11003521126760564No Hit
ACTTAAAAAAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC50.11003521126760564No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)