FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152736

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152736
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4809
Total Bases697.1 kbp
Sequences flagged as poor quality0
Sequence length141-145
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC147330.630068621335No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC101221.043876065710126No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG90618.83967560823456No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG2294.761904761904762No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG721.4971927635683093No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT591.2268662923684759No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT420.8733624454148471No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT420.8733624454148471No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT260.5406529423996673No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA220.4574755666458723No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTGG210.43668122270742354No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTGGCTAATGTAGAACCATGT170.35350384695362863No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA170.35350384695362863No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT140.2911208151382824No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA120.2495321272613849No Hit
AATACCGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT110.22873778332293615No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT110.22873778332293615No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA110.22873778332293615No Hit
AAATACCGTTCGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT90.18714909544603867No Hit
AAATACCGTTCAAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT90.18714909544603867No Hit
GGCACTAGAATGGACCTCTTCGTCATCACTAAATTATTTCAATTAAATAC80.16635475150758994No Hit
AAATACCGTTCAGAACTGCCTTGTTTTAAGTGGCTAATGTAGAACCATGT80.16635475150758994No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA80.16635475150758994No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCCTTGTTTTTAAGTGG70.1455604075691412No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTTCAAATTGCTGCTGGAACC70.1455604075691412No Hit
AAATACCGTTCAGAGCGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT70.1455604075691412No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAGTGGCTAATGTAGAACCATGT70.1455604075691412No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGATTGCAAATTGCTGCTGGAACC70.1455604075691412No Hit
AAATACCGTTAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT60.12476606363069245No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA60.12476606363069245No Hit
AAATACCGTTCAAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT60.12476606363069245No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCTTTGTTTTTAAGTGG60.12476606363069245Clontech SMARTer II A Oligonucleotide (95% over 22bp)
AAATACCGTTAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT60.12476606363069245No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCTTACCCACAGAAACA50.1039717196922437Clontech SMARTer II A Oligonucleotide (95% over 22bp)
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA50.1039717196922437No Hit
AAATACCGTTCAGCACTGCCTGTTTTTAAGTGGCTAATGTAGAACCATGT50.1039717196922437No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGACCC50.1039717196922437No Hit
AAATACCGTTCAGACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT50.1039717196922437No Hit
GGCACTAGAATGGATCTCTTCCTCATCACTAAATTATTTCAATTAAATAC50.1039717196922437No Hit
AAATACCGTTAAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC50.1039717196922437No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCCTGCAAATTGCTGCTGGAACC50.1039717196922437No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGAAAATTGCTGCTGGAACC50.1039717196922437No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATAGCTGCTGGAACC50.1039717196922437No Hit
AAATACCGTTCAGAGTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT50.1039717196922437No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTCGCAAATTGCTGCTGGAACC50.1039717196922437No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)