FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152744

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152744
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3255
Total Bases471.7 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC120436.98924731182795No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC62119.078341013824883No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG51815.913978494623656No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG1484.546850998463902No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT361.1059907834101383No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG250.7680491551459293No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA200.6144393241167435No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA190.5837173579109063No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT150.4608294930875576No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA150.4608294930875576No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA140.43010752688172044No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT140.43010752688172044No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA130.39938556067588327No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT130.39938556067588327No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA120.3686635944700461No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA110.3379416282642089No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA100.30721966205837176No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT70.21505376344086022No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAACAC70.21505376344086022No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTGGCTAATGTAGAACCATGT70.21505376344086022No Hit
AAATACCGTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT60.18433179723502305No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAGATAC60.18433179723502305No Hit
GGCACTAGAATGGACCCTTCCTCATCACTAAATTATTTCAATTAAATACA60.18433179723502305No Hit
GGCACTAGAATGGACCTCTTCGTCATCACTAAATTATTTCAATTAAATAC50.15360983102918588No Hit
AAATACCGTTAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT50.15360983102918588No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA50.15360983102918588No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGACCC50.15360983102918588No Hit
GGCACTAGAATGGACCTCTTCCTCACCACTAAATTATTTCAATTAAATAC50.15360983102918588No Hit
AAATACCGTTCAGAGCTGCCGCCAGGAGCTTGCAAATTGCTGCTGGAACC50.15360983102918588No Hit
GGCACTAGAATGGACTTCTTCCTCATCACTAAATTATTTCAATTAAATAC40.1228878648233487No Hit
AAATACCGTTCAGAGTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT40.1228878648233487No Hit
AATACCGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT40.1228878648233487No Hit
GGCACTAGAATGGACCTCTTCCCCATCACTAAATTATTTCAATTAAATAC40.1228878648233487No Hit
GGCACTAGAATGGCCTCTTCCTCATCACTAAATTATTTCAATTAAATACA40.1228878648233487No Hit
AAATACCGTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT40.1228878648233487No Hit
GGCACTAGAATGGACCTCCTCCTCATCACTAAATTATTTCAATTAAATAC40.1228878648233487No Hit
AAATACCGTTCAGAGCTCCCACCAGGAGCTTGCAAATTGCTGCTGGAACC40.1228878648233487No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTAAATTAAATAC40.1228878648233487No Hit
AAATACCGTTCAGAGTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT40.1228878648233487No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGAACT40.1228878648233487No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTAAATTATTTCAATTAAATAC40.1228878648233487No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTCCAATTAAATAC40.1228878648233487No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)