FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152760

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152760
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15198
Total Bases2.2 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC392025.792867482563498No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC360923.746545598105016No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG278018.2918805105935No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG6894.533491248848533No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC2641.7370706671930518No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT2301.5133570206606133No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC2241.4738781418607712No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG1891.2435846821950256No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT1110.7303592577970786No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT820.5395446769311751No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTGG700.46058691933149093No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT670.4408474799315699No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA610.40136860113172784No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTGGCTAATGTAGAACCATGT590.38820897486511385No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA560.3684695354651928No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA500.3289906566653507No Hit
AAATACCGTTCAGAGCTGCCATGTCATGAGTGGCCAGATGGAGAGGCCCA480.31583103039873667No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA340.22371364653243847No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA330.21713383339913148No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA310.20397420713251743No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA300.19739439399921044No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT240.15791551519936833No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA230.15133570206606134No Hit
AAATACCGTTCAGACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT220.14475588893275432No Hit
GGCACTAGAATGGACCTCTTCGTCATCACTACATTATTTCAATTAAATAC220.14475588893275432No Hit
AAATACCGTTAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT210.1381760757994473No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA210.1381760757994473No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGTAAATTGCTGCTGGAACC210.1381760757994473No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT200.13159626266614027No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCCTTGTTTTTAAGTGG190.12501644953283325No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA190.12501644953283325No Hit
AAATACCGTTAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT190.12501644953283325No Hit
AAATACGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT180.11843663639952626No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAGTGGCTAATGTAGAACCATGT180.11843663639952626No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGATGCTGGAACC170.11185682326621924No Hit
AATACCGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT170.11185682326621924No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGACCC170.11185682326621924No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCTTACCCACAGAAACA160.10527701013291224Clontech SMARTer II A Oligonucleotide (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)