FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152762

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152762
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15950
Total Bases2.3 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC263716.53291536050157No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC257416.137931034482758No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG243115.241379310344827No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC242215.184952978056426No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC15949.993730407523511No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG4332.7147335423197494No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT2431.5235109717868338No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG1220.7648902821316614No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT1070.670846394984326No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT600.3761755485893417No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA530.3322884012539185No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA490.3072100313479624No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTGGCTAATGTAGAACCATGT480.3009404388714733No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT470.2946708463949843No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT440.27586206896551724No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTGG370.23197492163009406No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA360.22570532915360503No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA350.219435736677116No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA320.2006269592476489No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA290.18181818181818182No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA270.16927899686520376No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA270.16927899686520376No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA260.16300940438871472No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA260.16300940438871472No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA250.1567398119122257No Hit
AAATACGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT250.1567398119122257No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA250.1567398119122257No Hit
AAATACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT220.13793103448275862No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA200.12539184952978058No Hit
GGCACTAGAATGGACCTCTTCGTCATCACTAAATTATTTCAATTAAATAC190.11912225705329155No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA190.11912225705329155No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA170.10658307210031348No Hit
GGCACTAGAATGGACCTCTTCGTCATCACTACATTATTTCAATTAAATAC160.10031347962382445No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA160.10031347962382445No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)