FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152766

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152766
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11861
Total Bases1.7 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC377131.79327206812242No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG184315.538318860129838No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC144612.191214905994435No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC10218.608043166680718No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG4553.836101509147627No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC3122.6304696062726585No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT2331.9644212123766966No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG1431.2056319028749685No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT1401.1803389258915775No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA770.6491864092403676No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTGG580.4889975550122249No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA530.44684259337324No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT480.4046876317342551No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT460.3878256470786612No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA460.3878256470786612No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA440.3709636624230672No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTGGCTAATGTAGAACCATGT380.32037770845628527No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT350.2950847314728944No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA300.25292976983390947No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA290.24449877750611246No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA290.24449877750611246No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA270.22763679285051852No Hit
GGCACTAGAATGGACCTCTTCGTCATCACTACATTATTTCAATTAAATAC250.21077480819492456No Hit
AAATACCGTTCAGACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT190.16018885422814264No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA170.1433268695725487No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA160.13489587724475172No Hit
AAATACGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT160.13489587724475172No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTACATTATTTCAATTAAATAC160.13489587724475172No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCTTACCCACAGAAACA130.10960290026136076Clontech SMARTer II A Oligonucleotide (95% over 22bp)
AAATACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT130.10960290026136076No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA120.10117190793356377No Hit
AAATACCGTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT120.10117190793356377No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)