FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152768

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152768
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16323
Total Bases2.3 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC485729.755559639772102No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG294718.054279237885194No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC276116.91478282178521No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC12097.4067267046498815No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG7204.410953868774122No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG2121.2987808613612695No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA870.5329902591435398No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT830.5084849598725725No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT690.4227164124241867No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA650.3982111131532194No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTGG580.35532683942902654No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTGGCTAATGTAGAACCATGT580.35532683942902654No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA550.33694786497580104No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT550.33694786497580104No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA530.32469521534031737No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA470.28793726643386636No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA440.26955829198064085No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA420.2573056423451571No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA420.2573056423451571No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT350.21442136862096428No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA310.18991606934999694No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT300.1837897445322551No Hit
GGCACTAGAATGGACCTCTTCGTCATCACTACATTATTTCAATTAAATAC270.1654107700790296No Hit
AAATACGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT230.14090547080806223No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA230.14090547080806223No Hit
GGCACTAGAATGGACCCTTCCTCATCACTACATTATTTCAATTAAATACA230.14090547080806223No Hit
AAATACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT220.13477914599032043No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTACATTATTTCAATTAAATAC200.12252649635483674No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAGTGGCTAATGTAGAACCATGT200.12252649635483674No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCCTTGTTTTTAAGTGG190.11640017153709489No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA190.11640017153709489No Hit
AAATACCGTTAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT190.11640017153709489No Hit
AAATACCGTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT190.11640017153709489No Hit
AAATACCGTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT180.11027384671935306No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGACCC180.11027384671935306No Hit
AAATACCGTTAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT180.11027384671935306No Hit
AATACCGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT170.10414752190161121No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT170.10414752190161121No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACAGGCTAATGTAGAA170.10414752190161121No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)