FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152778

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152778
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10450
Total Bases1.5 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC342232.74641148325359No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC245823.52153110047847No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG175216.76555023923445No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG5435.196172248803828No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG920.8803827751196172No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA720.6889952153110047No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA500.4784688995215311No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTGGCTAATGTAGAACCATGT480.45933014354066987No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA460.4401913875598086No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT420.40191387559808617No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA410.39234449760765555No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA410.39234449760765555No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA390.37320574162679426No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA380.36363636363636365No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA340.3253588516746412No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA320.3062200956937799No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA290.27751196172248804No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA250.23923444976076555No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA250.23923444976076555No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA240.22966507177033493No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA240.22966507177033493No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA240.22966507177033493No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTGG220.21052631578947367No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA180.17224880382775118No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA180.17224880382775118No Hit
AAATACCGTTCAAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT160.15311004784688995No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA150.14354066985645933No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA150.14354066985645933No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTACATTATTTCAATTAAATAC130.1244019138755981No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCTTACCCACAGAAACA120.11483253588516747Clontech SMARTer II A Oligonucleotide (95% over 22bp)
GGCACTAGAATGGACCTCTTCGTCATCACTAAATTATTTCAATTAAATAC120.11483253588516747No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAATGGCTAATGTAGAACCATGT120.11483253588516747No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAAATAAATAC120.11483253588516747No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA120.11483253588516747No Hit
AAATACCGTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT120.11483253588516747No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA120.11483253588516747No Hit
AAATACGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT110.10526315789473684No Hit
GGCACTAGAATGGACCTCTTCGCCAAGCAAACTGGACCCACAGAAACACA110.10526315789473684No Hit
AATACCGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT110.10526315789473684No Hit
GGCACTAGAATGGACCTCTTCCTCATAACTACATTATTTCAATTAAATAC110.10526315789473684No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)