FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152809

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152809
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3847
Total Bases557.7 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAAAAACAAGGCAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCTC103026.774109695866912No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCT90123.420847413569014No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTTGGACAGACCAAGTCTC86522.48505328827658No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTTTGGACAGACCAAGTCTC2055.328827657915259No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG1393.6132050948791266No Hit
ACTTAAAAACAAGGCAGTGCTGAACGGTATTTTTGGACAGACCAAGTCTC511.3257083441642838No Hit
ACTTAAAACAAGGCAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCTCT300.7798284377436964No Hit
TGTGTGTGTGTTTCTGTGGGTCCAGTTTGCTTGGCGTAGAGGTCCATTCT290.7538341564855732No Hit
ACTTAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC240.6238627501949572No Hit
TGTGTGTGTGTTTCTGTGGGTCCAGTTTGCTTGGCGAAGAGGTCCATTCT200.5198856251624643No Hit
ACTTAAAACAAGGCAGCTCTGAACGGTATTTTTGGACAGACCAAGTCTCT160.4159085001299714No Hit
ACTTAAAAACAGGCAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCTCT160.4159085001299714No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTGGACAGACCAAGTCTCT120.3119313750974786No Hit
ACTTAAAAACAAGGCAGTGCTGTGCCCTCCCAGGTACCCCAAGCTGGCAG110.28593709383935534No Hit
ACTTAAAAACAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC110.28593709383935534No Hit
TGTGTGTGTGTTTCTGTGGGGCCAGTTTGCTTGGCGTAGAGGTCCATTCT100.25994281258123214No Hit
ACTTAAAAACAAGGCAGTCTGAACGGTATTTTTGGACAGACCAAGTCTCT100.25994281258123214No Hit
ACTTAAAAACAAGGCAGCTCTGACGGTATTTTTGGACAGACCAAGTCTCT100.25994281258123214No Hit
ACTAAAAACAAGGCAGCTCTGAACGGTATTTTTGGACAGACCAAGTCTCT100.25994281258123214No Hit
ACTTAAAAACAAGGCAGCTCTGAACGTATTTTTGGACAGACCAAGTCTCT90.23394853132310892No Hit
ACTAAAAACAAGGCAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCTCT80.2079542500649857No Hit
ACTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC80.2079542500649857No Hit
ATTAAAAACAAGGCAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCTCT70.1819599688068625No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCC60.1559656875487393No Hit
ACTTAAAAACAAGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC50.12997140629061607No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTTGGACAGACCAAGTCTCT50.12997140629061607No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTACTACAGTTTCTGCCT50.12997140629061607No Hit
TGTGTGTGTGTTTCTGTGGGCCAGTTTGCTTGGCGTAGAGGTCCATTCTA50.12997140629061607No Hit
TGTGTGTGTGTTTCTGTGGGGCCAGTTTGCTTGGCGAAGAGGTCCATTCT50.12997140629061607No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCCGCCT50.12997140629061607No Hit
ACTTAAAAACAAGGCAGTGCTAATGCTAATGGTGCTACAGTTCCTGCCTC50.12997140629061607No Hit
ATTAAAAACAAGGCAGCTCTGAACGGTATTTTTGGACAGACCAAGTCTCT50.12997140629061607No Hit
CTTAAAAACAAGGCAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCTCT40.10397712503249285No Hit
ACTTAAAAACAAGCAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCTCT40.10397712503249285No Hit
ACTTAAAAACAAGGCAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCCC40.10397712503249285No Hit
ACTTAAAAACAAGGCAGCTCTAACGGTATTTTTGGACAGACCAAGTCTCT40.10397712503249285No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTTGGACAGACAAAGTCTC40.10397712503249285No Hit
TGTGTGTGTGTTTCTGTGGGCCCAGTTTGCTTGGCGTAGAGGTCCATTCT40.10397712503249285No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)