FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152820

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152820
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10720
Total Bases1.5 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC180416.828358208955223No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC179816.77238805970149No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG173716.203358208955223No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC126211.772388059701493No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC115510.774253731343283No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG4243.955223880597015No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT1331.2406716417910448No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG1060.9888059701492538No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT580.541044776119403No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT580.541044776119403No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT340.3171641791044776No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA330.3078358208955224No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTGG290.2705223880597015No Hit
AAATACCGTTCAGAGCTGCCATGTCATGAGTGGCCAGATGGAGAGGCCCA270.251865671641791No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA260.2425373134328358No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTGGCTAATGTAGAACCATGT250.23320895522388058No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA240.22388059701492538No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA230.21455223880597016No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT230.21455223880597016No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA220.2052238805970149No Hit
AAATACGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT190.17723880597014927No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGAAAGCCAATGGGAAA190.17723880597014927No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA190.17723880597014927No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA180.16791044776119401No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA170.1585820895522388No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA160.1492537313432836No Hit
AAATACCGTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT160.1492537313432836No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA150.13992537313432835No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA150.13992537313432835No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA130.1212686567164179No Hit
AAATACCGTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT130.1212686567164179No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA130.1212686567164179No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA130.1212686567164179No Hit
AAATACCGTTCAGAGCTGCCATGTCATGAGTGGCCAGATAGAGAGGCCCA130.1212686567164179No Hit
AAATACCGTTCAGAGTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT130.1212686567164179No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA120.11194029850746269No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA120.11194029850746269No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT120.11194029850746269No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA110.10261194029850745No Hit
AAATACCGTTCAGACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT110.10261194029850745No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)