FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152821

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152821
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3162
Total Bases458.3 kbp
Sequences flagged as poor quality0
Sequence length140-145
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAAAAACAAGGCAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCTC113335.83175205566098No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTTGGACAGACCAAGTCTC103032.57432005060089No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTTTGGACAGACCAAGTCTC2798.823529411764707No Hit
ACTTAAAAACAAGGCAGTGCTGAACGGTATTTTTGGACAGACCAAGTCTC912.8779253636938646No Hit
ACTTAAAAACAAGGCAGTGCTGTGCCCTCCCAGGTACCCCAAGCTGGCAG471.4864010120177102No Hit
ACTTAAAACAAGGCAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCTCT321.0120177103099304No Hit
ACTTAAAACAAGGCAGCTCTGAACGGTATTTTTGGACAGACCAAGTCTCT230.7273877292852625No Hit
ACTTAAAAACAAGGCAGTGCTATGCCCTCCCAGGTACCCCAAGCTGGCAG230.7273877292852625No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTGGACAGACCAAGTCTCT200.6325110689437065No Hit
ACTTAAAAACAAGGCAGTGCTATCAACGAAGAGGTCCATTCTAGTGCCTT150.47438330170777987No Hit
ACTAAAAACAAGGCAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCTCT150.47438330170777987No Hit
ACTTAAAAACAGGCAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCTCT90.2846299810246679No Hit
ACTTAAAAAAAGGCAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCTCT80.2530044275774826No Hit
ACTTAAAAACAAGGCAGCTCTAACGGTATTTTTGGACAGACCAAGTCTCT80.2530044275774826No Hit
ACTTAAAAACAAGGCGGTGCTAATGCTAATGGTGCTACAGTTTCTGCCTC70.2213788741302973No Hit
ACTTAAAAACAAGGCAGCTCGAACGGTATTTTTGGACAGACCAAGTCTCT60.18975332068311196No Hit
ACTTAAAAACAAGGCAGTGCTAATGCTAATGGTGCTACAGTTTCCGCCTC60.18975332068311196No Hit
ACTTAAAAACAAGGCAGTGGTATCAACGAAGAGGTCCATTCTAGTGCCTT60.18975332068311196No Hit
ACTTAAAAACAAGGCAGCTCTGAACGTATTTTTGGACAGACCAAGTCTCT60.18975332068311196No Hit
CTTAAAAACAAGGCAGCTCTGAACGGTATTTTTGGACAGACCAAGTCTCT60.18975332068311196No Hit
ACTTAAAAACAAGCAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCTCT50.15812776723592661No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTTTTTTGGACAGACCAAGTCTCT50.15812776723592661No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTTGGACAGACCAAGTCTCT50.15812776723592661No Hit
ACTTAAAAACAAGGCAGTGCTGATGCTAATGGTGCTACAGTTTCTGCCTC50.15812776723592661No Hit
ACTTAAAAACAAGGCAGTGCTAATGCTAATGGCGCTACAGTTTCTGCCTC40.1265022137887413No Hit
ACTTAAAAACAAGGCAGTTCTAATGCTAATGGTGCTACAGTTTCTGCCTC40.1265022137887413No Hit
ACTTAAAAACAAGGCAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCCC40.1265022137887413No Hit
ACTTAAAAACAAGGCAGCTCTGAAGGTATTTTTGGACAGACCAAGTCTCT40.1265022137887413No Hit
ACTTAAAAACAAGGCAGTGCTAATGCTAATGGTGCCACAGTTTCTGCCTC40.1265022137887413No Hit
ACTTAAAAACAGGCAGCTCTGAACGGTATTTTTGGACAGACCAAGTCTCT40.1265022137887413No Hit
ATTAAAAACAAGGCAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCTCT40.1265022137887413No Hit
ACTTAAAAACAAGGCAGTTCTGAACAGTATTTTTGGACAGACCAAGTCTC40.1265022137887413No Hit
ACTAAAAACAAGGCAGCTCTGAACGGTATTTTTGGACAGACCAAGTCTCT40.1265022137887413No Hit
ACTTAAAAACAAGGCAGTGCTAGTGCTAATGGTGCTACAGTTTCTGCCTC40.1265022137887413No Hit
ACTTAAAACAAGGCAGTTCTGAACGGTATTTTTGGACAGACCAAGTCTCT40.1265022137887413No Hit
ACTTAAAAACAAGGCACTCTGAACGGTATTTTTGGACAGACCAAGTCTCT40.1265022137887413No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)