FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152822

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152822
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3162
Total Bases458.4 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC53

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC105133.23845667299178No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG101432.06831119544592No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG2758.697027197975965No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG862.719797596457938No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT481.5180265654648957No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT240.7590132827324478No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTGGCTAATGTAGAACCATGT220.6957621758380772No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT220.6957621758380772No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT200.6325110689437065No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTGG150.47438330170777987No Hit
AAATACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT110.3478810879190386No Hit
AAATACCGTTCAAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT100.31625553447185323No Hit
AAATACCGTTCAGAGTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT90.2846299810246679No Hit
AAAACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT80.2530044275774826No Hit
AAATACCGTTAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT80.2530044275774826No Hit
AAATACGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT60.18975332068311196No Hit
AAATACCGTTAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT60.18975332068311196No Hit
AAATACCGTTCGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT60.18975332068311196No Hit
AAATACCTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT60.18975332068311196No Hit
AAATACCGTTCGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT60.18975332068311196No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGGGCTAATGTAGAACCATGT60.18975332068311196No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGACCC60.18975332068311196No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCCTTGTTTTTAAGTGG50.15812776723592661No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCAAG50.15812776723592661No Hit
AAAACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT50.15812776723592661No Hit
AAATACCGTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT50.15812776723592661No Hit
AATACCGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT40.1265022137887413No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTTCAAATTGCTGCTGGAACC40.1265022137887413No Hit
AAATACCTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT40.1265022137887413No Hit
AAATACTGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG40.1265022137887413No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC40.1265022137887413No Hit
AAATACCGTTCAGAGTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT40.1265022137887413No Hit
AAATACCGTTCAGAGCTGCCTGTTTTTAAGTGGCTAATGTAGAACCATGT40.1265022137887413No Hit
AAATACCGTTCAGGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT40.1265022137887413No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAGTGGCTAATGTAGAACCATGT40.1265022137887413No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)