FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152851

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152851
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12558
Total Bases1.8 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG571545.50883898709986No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTTGGACAGACCAAGTCTC196115.615543876413444No Hit
ACTTAAAAACAAGGCAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCTC138010.989010989010989No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCT11198.910654562828475No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTTTGGACAGACCAAGTCTC6014.785793916228699No Hit
ACTTAAAAACAAGGCAGTGCTGAACGGTATTTTTGGACAGACCAAGTCTC970.7724159898072942No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTGGACAGACCAAGTCTCT520.4140786749482402No Hit
ACTTAAAACAAGGCAGCTCTGAACGGTATTTTTGGACAGACCAAGTCTCT440.3503742634177417No Hit
ACTTAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC390.3105590062111801No Hit
ACTTAAAACAAGGCAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCTCT370.2946329033285555No Hit
ACTTAAAAACAAGGCAGTGCTGTGCCCTCCCAGGTACCCCAAGCTGGCAG350.2787068004459309No Hit
TGTGTGTGTGTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC350.2787068004459309No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAGG250.19907628603280775No Hit
TGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGCT220.17518713170887085No Hit
ACTTAAAAACAAGGCAGTGCTATCAACGAAGAGGTCCATTCTAGTGCCTT220.17518713170887085No Hit
ACTTAAAAACAAGGCAGCTCTGAACGTATTTTTGGACAGACCAAGTCTCT200.15926102882624624No Hit
TGTGTGTGTGTTTTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC190.1512979773849339No Hit
ACTTAAAAACAAGGCAGCTCTGACGGTATTTTTGGACAGACCAAGTCTCT180.1433349259436216No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTTGGACAGACCAAGTCTCT170.13537187450230928No Hit
ACTTAAAAACAAGGCAGTCTGAACGGTATTTTTGGACAGACCAAGTCTCT170.13537187450230928No Hit
TGTGTGTGTGTTTCGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC150.11944577161968467No Hit
TGTGTGGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC140.11148272017837235No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAGAG140.11148272017837235No Hit
TGTGTGTGTTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC130.10351966873706005No Hit
ACTTAAAAACAGGCAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCTCT130.10351966873706005No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)