FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152852

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152852
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12558
Total Bases1.8 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC450235.84965758878803No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG192715.344800127408822No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC127310.136964484790571No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC10158.082497212931996No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG5914.706163401815576No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC5694.530976270106705No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG970.7724159898072942No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA890.7087115782767957No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA510.40611562350692787No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA510.40611562350692787No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT500.3981525720656155No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA480.3822264691829909No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA470.3742634177416786No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTGGCTAATGTAGAACCATGT430.3424112119764294No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA420.33444816053511706No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA380.3025959547698678No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT370.2946329033285555No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA300.23889154323936934No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT270.21500238891543239No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA250.19907628603280775No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT240.19111323459149546No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTGG200.15926102882624624No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA200.15926102882624624No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA190.1512979773849339No Hit
GGCACTAGAATGGACCCTTCCTCATCACTACATTATTTCAATTAAATACA190.1512979773849339No Hit
AAATACGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT180.1433349259436216No Hit
AAATACCGTTCAGACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT170.13537187450230928No Hit
AAATACCGTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT170.13537187450230928No Hit
GGCACTAGAATGGACCTCTTCGTCATCACTACATTATTTCAATTAAATAC160.12740882306099696No Hit
AATACCGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT160.12740882306099696No Hit
AAATACCGTTAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT140.11148272017837235No Hit
AAATACCGTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT140.11148272017837235No Hit
GGCACTAGAATGGCCTCTTCCTCATCACTACATTATTTCAATTAAATACA140.11148272017837235No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTACATTATTTCAATTAAATAC130.10351966873706005No Hit
AAATACCGTTAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT130.10351966873706005No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)