FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152858

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152858
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8348
Total Bases1.2 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC228227.335888835649257No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC187022.400574988021084No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG116613.967417345471969No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC6888.241494968854814No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG2943.5218016291327268No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC1321.581217057977959No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG1191.4254911356013418No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT630.7546717776712986No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT450.5390512697652132No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA400.4791566842357451No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT370.4432199329180642No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT340.4072831816003833No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTGG300.3593675131768088No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA260.3114518447532343No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA240.287494010541447No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA230.2755150934355534No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA210.2515572592237662No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA210.2515572592237662No Hit
AAATACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT210.2515572592237662No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTGGCTAATGTAGAACCATGT170.20364159080019165No Hit
AAATACCGTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT170.20364159080019165No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA160.19166267369429804No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA140.16770483948251078No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGACCC140.16770483948251078No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT130.15572592237661714No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACGTTATTTCAATTAAATAC120.1437470052707235No Hit
AAATACCGTTAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT120.1437470052707235No Hit
AAATACCGTTCAGACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT110.1317680881648299No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA100.11978917105893627No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGATGCTGGAACC100.11978917105893627No Hit
AAATACCGTTCAGAGTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT100.11978917105893627No Hit
AAATCCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT90.10781025395304264No Hit
AAATACCGTTCAGGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT90.10781025395304264No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)