FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152866

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152866
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2993
Total Bases433.8 kbp
Sequences flagged as poor quality0
Sequence length141-145
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC86728.96759104577347No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC84928.366187771466755No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG35311.794186435015035No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC1424.7444036084196455No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG842.8065486134313398No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG290.968927497494153No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTGG220.7350484463748747No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT180.6014032743067156No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA140.4677581022385567No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA140.4677581022385567No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA130.4343468092215168No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT130.4343468092215168No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT120.4009355162044771No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT110.3675242231874373No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTGGCTAATGTAGAACCATGT110.3675242231874373No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA100.3341129301703976No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT80.2672903441363181No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT80.2672903441363181No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA70.23387905111927834No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA70.23387905111927834No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGATGCTGGAACC60.20046775810223855No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA60.20046775810223855No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCCGGAACC60.20046775810223855No Hit
AAATCCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT50.1670564650851988No Hit
AAATACCGTTCAGAGCTGCCCCCAGGAGCTTGCAAATTGCTGCTGGAACC50.1670564650851988No Hit
AAATACCGTTAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT50.1670564650851988No Hit
AAATACCGTTCAAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT50.1670564650851988No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT50.1670564650851988No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGACCC50.1670564650851988No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTAAATTATTTCAATTAAATAC50.1670564650851988No Hit
AAATACCGTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT40.13364517206815904No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA40.13364517206815904No Hit
GGCACTAGAATGGACCTCTTCGTCATCACTAAATTATTTCAATTAAATAC40.13364517206815904No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA40.13364517206815904No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC40.13364517206815904No Hit
AAATACCGTTCAGACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT40.13364517206815904No Hit
AAATACCGTTCGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT40.13364517206815904No Hit
GGCACTAGAATGGACCCTTCCTCATCACTAAATTATTTCAATTAAATACA40.13364517206815904No Hit
GGCACTAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACAA30.10023387905111927No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAAATAAATAC30.10023387905111927No Hit
AAATACCGCTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC30.10023387905111927No Hit
AAATACCGTTCAGAGCTGACACCAGGAGCTTGCAAATTGCTGCTGGAACC30.10023387905111927No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGATGGAACC30.10023387905111927No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTTGAACC30.10023387905111927No Hit
AAATACCGTTCAGAGCTGCCACCGGGAGCTTGCAAATTGCTGCTGGAACC30.10023387905111927No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAGTAC30.10023387905111927No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA30.10023387905111927No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCAGGAACC30.10023387905111927No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTTCTGCTGGAACC30.10023387905111927No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCCGCTGGAACC30.10023387905111927No Hit
AAATACCGTTCAGAGCTGCCTCCAGGAGCTTGCAAATTGCTGCTGGAACC30.10023387905111927No Hit
AAATACCGTTCAAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT30.10023387905111927No Hit
GGCACTAGAATGGACCTCTTCCTCGTCACTAAATTATTTCAATTAAATAC30.10023387905111927No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCAGCTGGAACC30.10023387905111927No Hit
AAATACCGTTCAGAGCTGCCACAAGGAGCTTGCAAATTGCTGCTGGAACC30.10023387905111927No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGATTGCAAATTGCTGCTGGAACC30.10023387905111927No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)