FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152870

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152870
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1746
Total Bases253 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC39822.79495990836197No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC36921.1340206185567No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG28616.38029782359679No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC17910.252004581901488No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG844.810996563573884No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG221.2600229095074456No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC211.202749140893471No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT130.7445589919816723No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT110.6300114547537228No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT100.572737686139748No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA60.3436426116838488No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA60.3436426116838488No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT60.3436426116838488No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAAATAAATAC40.22909507445589922No Hit
AAATACCGTTAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT40.22909507445589922No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA40.22909507445589922No Hit
AAATACCGTTCGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT40.22909507445589922No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA40.22909507445589922No Hit
AAATACCTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT40.22909507445589922No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTGGCTAATGTAGAACCATGT40.22909507445589922No Hit
AAATACCGTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT30.1718213058419244No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA30.1718213058419244No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA30.1718213058419244No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATGC30.1718213058419244No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTGG30.1718213058419244No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA30.1718213058419244No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA30.1718213058419244No Hit
AATACCGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT30.1718213058419244No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT30.1718213058419244No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTAAATTAAATAC30.1718213058419244No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA30.1718213058419244No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATATCAATTAAATAC30.1718213058419244No Hit
GGCACTAGAATGGACCCCTTCCTCATCACTACATTATTTCAATTAAATAC20.11454753722794961No Hit
AGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC20.11454753722794961No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCCTTGTTTTTAAGTGG20.11454753722794961No Hit
GGCACTAAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTGGC20.11454753722794961No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA20.11454753722794961No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCTTACCCACAGAAACA20.11454753722794961Clontech SMARTer II A Oligonucleotide (95% over 22bp)
GAATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG20.11454753722794961No Hit
AAATACCGTTCAGAGCTCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT20.11454753722794961No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA20.11454753722794961No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGTAACC20.11454753722794961No Hit
AAATACCTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT20.11454753722794961No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGGACC20.11454753722794961No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGGGCTAATGTAGAACCATGT20.11454753722794961No Hit
GGCACTAGAATGGCCTCTTCCTCATCACTAAATTATTTCAATTAAATACA20.11454753722794961No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATCTCAATTAAATAC20.11454753722794961No Hit
AAATACCGTTCAGAGCTGCCATGTCATGAGTGGCCAGATAGAGAGGCCCA20.11454753722794961No Hit
GGCACTAGAATGGACCTCTTCCTCATCGCTACATTATTTCAATTAAATAC20.11454753722794961No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA20.11454753722794961No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTCGCAAATTGCTGCTGGAACC20.11454753722794961No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGGGGCTAATGTAGAACCATG20.11454753722794961No Hit
GGCACTAGAATGGACCTTTTCCTCATCACTACATTATTTCAATTAAATAC20.11454753722794961No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA20.11454753722794961No Hit
AAATACCGTTCAGAGCTGCCACCAGGGGCTTGCAAATTGCTGCTGGAACC20.11454753722794961No Hit
AGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC20.11454753722794961No Hit
AATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACTG20.11454753722794961No Hit
AATCCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGTC20.11454753722794961No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)