FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152891

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152891
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1742
Total Bases252.5 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG76744.02985074626866No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCT40323.134328358208954No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTTGGACAGACCAAGTCTC22913.14580941446613No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTTTGGACAGACCAAGTCTC804.5924225028702645No Hit
ACTTAAAAACAAGGCAGTGCTGAACGGTATTTTTGGACAGACCAAGTCTC201.1481056257175661No Hit
ACTTAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC120.6888633754305395No Hit
TGTGTGTGTGTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC90.5166475315729048No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTGGACAGACCAAGTCTCT80.4592422502870264No Hit
ACTTAAAAAAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC60.3444316877152698No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGGAGGGTAAAG50.28702640642939153No Hit
ACTTAAAACAAGGCAGCTCTGAACGGTATTTTTGGACAGACCAAGTCTCT50.28702640642939153No Hit
ACTTAAAAACAAGGAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC50.28702640642939153No Hit
ACTTAAAAACAAGGCGGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCT40.2296211251435132No Hit
ACTAAAAACAAGGCAGCTCTGAACGGTATTTTTGGACAGACCAAGTCTCT40.2296211251435132No Hit
ACTTAAAAACAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC40.2296211251435132No Hit
ACTTAAAAACAAGGCAGTGTTAATGCCTAATGGTGCTACAGTTTCTGCCT30.1722158438576349No Hit
ACTTAAAAACAAGGCAGCTCTAACGGTATTTTTGGACAGACCAAGTCTCT30.1722158438576349No Hit
ACTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC30.1722158438576349No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGCAAAG30.1722158438576349No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAGGGGTAAAG30.1722158438576349No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAGGGTTTGGACAGAAGGGTAAAG30.1722158438576349No Hit
TGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGCT30.1722158438576349No Hit
ACTTAAAACAAGGCAGTGCTGAACGGTATTTTTGGACAGACCAAGTCTCT20.1148105625717566No Hit
TGTGTGTGTGTTTCTGTGGGTCCAGTTTGCTTGGCGTAGAAGAGGTCCAT20.1148105625717566No Hit
TGTGTGTGTGTTTCCGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG20.1148105625717566No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGAGTAAAG20.1148105625717566No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGATAAAG20.1148105625717566No Hit
TGTGTGTGTGTTTCGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC20.1148105625717566No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAGAG20.1148105625717566No Hit
ACTTAAAAACAAGGCAGCTCTGAACGTATTTTTGGACAGACCAAGTCTCT20.1148105625717566No Hit
CTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC20.1148105625717566No Hit
TGTGTGTGGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC20.1148105625717566No Hit
ACTTAAAAACAAGGCAGTCTGAACGTATTTTTGGACAGACCAAGTCTCTG20.1148105625717566No Hit
ACTTAAAAACAAGGCAGCTCTGAAGGTATTTTTGGACAGACCAAGTCTCT20.1148105625717566No Hit
ACTTAAAAACAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA20.1148105625717566Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTTAAAAACAAGGCAGTGCTATGCCCTCCCAGGTACCCCAAGCTGGCAG20.1148105625717566No Hit
ACTTAAAAACAAGGCGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC20.1148105625717566No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGATTTTTGGACAGACCAAGTCTCT20.1148105625717566No Hit
TGTGCGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG20.1148105625717566No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACGGTTTCTGCCT20.1148105625717566No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAA20.1148105625717566No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGCTTGGACAGAAGGGTAAAG20.1148105625717566No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGGCAGAAGGGTAAAG20.1148105625717566No Hit
TGGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC20.1148105625717566No Hit
TGTGTGTGTGTTTCTGTGGGTTCCTTTAAGGTTTGGACAGAAGGGTAAAG20.1148105625717566No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)