FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152904

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152904
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1708
Total Bases247.6 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC49228.805620608899297No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG39122.892271662763466No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC31218.26697892271663No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG1116.498829039812646No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG311.8149882903981265No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT170.9953161592505856No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT140.819672131147541No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTGGCTAATGTAGAACCATGT110.6440281030444965No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT90.5269320843091335No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA70.4098360655737705No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT70.4098360655737705No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT70.4098360655737705No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA70.4098360655737705No Hit
AAATACCGTTCAGACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT60.351288056206089No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA50.29274004683840754No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGATGCTGGAACC40.234192037470726No Hit
AAAACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT40.234192037470726No Hit
AAATACGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT40.234192037470726No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGGGCTAATGTAGAACCATGT40.234192037470726No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA40.234192037470726No Hit
AAATACCGTTCAGAACTGCCTTGTTTTAAGTGGCTAATGTAGAACCATGT40.234192037470726No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAGCC30.1756440281030445No Hit
AAATACCGTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT30.1756440281030445No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA30.1756440281030445No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA30.1756440281030445No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGGACC30.1756440281030445No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGACCC30.1756440281030445No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTAGCAAATTGCTGCTGGAACC30.1756440281030445No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGATTGCAAATTGCTGCTGGAACC30.1756440281030445No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCTTCCATGTACTCTGC30.1756440281030445Clontech SMARTer II A Oligonucleotide (95% over 22bp)
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA20.117096018735363No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGCACC20.117096018735363No Hit
AAATACCGTTCAGAGCTGCCTTGATTTTAAGTGGCTAATGTAGAACCATG20.117096018735363No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA20.117096018735363No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTATATACA20.117096018735363No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCATGCAAATTGCTGCTGGAACC20.117096018735363No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA20.117096018735363No Hit
AAATCCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT20.117096018735363No Hit
AAATACCGTTCAGAGCTGCCCCCAGGAGCTTGCAAATTGCTGCTGGAACC20.117096018735363No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGAAAATTGCTGCTGGAACC20.117096018735363No Hit
AAATACCGTTAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT20.117096018735363No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTACATTATTTCAATTAAATAC20.117096018735363No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA20.117096018735363No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATAGCTGCTGGAACC20.117096018735363No Hit
AAATACCGTTCAGAGTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT20.117096018735363No Hit
GGCACTAGAATGGACCTCTTCCTCATAACTACATTATTTCAATTAAATAC20.117096018735363No Hit
GGCACTAGAATGGACCTCGTCCTCATCACTACATTATTTCAATTAAATAC20.117096018735363No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTTCTGGAACC20.117096018735363No Hit
AAATACCGTTCAGAGTTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC20.117096018735363No Hit
AAAAACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC20.117096018735363No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTTCTGCTGGAACC20.117096018735363No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCCGCTGGAACC20.117096018735363No Hit
AAATACCGTTCAGAGTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT20.117096018735363No Hit
AAATACCGTTCAGAGCCGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG20.117096018735363No Hit
AAATACCGTTCAGAGCTGCCTTGTTTAAGTGGCTAATGTAGAACCATGTC20.117096018735363No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAGTGGCTAATGTAGAACCATGT20.117096018735363No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC20.117096018735363No Hit
AAATACCGTTCAAGCTGCCTTGTTTTAAGTGGCTAATGTAGAACCATGTC20.117096018735363No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)