FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152924

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152924
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences659
Total Bases95.5 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG15924.12746585735964No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC12518.968133535660094No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC12418.81638846737481No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG619.256449165402124No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG162.4279210925644916No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC111.669195751138088No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT71.062215477996965No Hit
GGCACTAGAATGGACCTCTTAGCACTGCCTTGTTTTTAAGTGGCTAATGT50.7587253414264037No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTGG40.6069802731411229No Hit
AAATACCGTTAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT40.6069802731411229No Hit
AAATACCGTTCAGACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT30.4552352048558422No Hit
AAATACCGTTCAGAGCTGCCATGTCATGAGTGGCCAGATGGAGAGGCCCA30.4552352048558422No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT30.4552352048558422No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAATGGCTAATGTAGAACCATGT20.30349013657056145No Hit
GGCACTAGAATGGATCTCTTCCTCATCACTACATTATTTCAATTAAATAC20.30349013657056145No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA20.30349013657056145No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA20.30349013657056145No Hit
AAATACCGTTCAGAACTGCCTTGTTTTAAGTGGCTAATGTAGAACCATGT20.30349013657056145No Hit
AAATACCGTTCAGAGCTGCCCCCAGGAGCTTGCAAATTGCTGCTGGAACC20.30349013657056145No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT20.30349013657056145No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA20.30349013657056145No Hit
AATACCGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT20.30349013657056145No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTGGCTAATGTAGAACCATGT20.30349013657056145No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGGACC20.30349013657056145No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCTCCCATGTACTCTGC20.30349013657056145Clontech SMARTer II A Oligonucleotide (95% over 21bp)
AAATACCGTTCAGAGCTGCCAACAGGATAATTCTATTAGCTGCTGGCAGC10.15174506828528073No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT10.15174506828528073No Hit
AAATACGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACATGTC10.15174506828528073No Hit
AAATACCGTTAAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG10.15174506828528073No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAGCC10.15174506828528073No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCAGT10.15174506828528073No Hit
AAATACGTTCAGAGCTGCATTGTTTTTAAGTGGCTAATGTAGAACCATGT10.15174506828528073No Hit
AAATACTGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG10.15174506828528073No Hit
AAATACCGTTCAGAACAGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG10.15174506828528073No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAAACATG10.15174506828528073No Hit
AAATACCGATCAGAGCAGCCACCAGGAGCTTCCAAAATCCAGCTCGAACA10.15174506828528073No Hit
GGCACTAGAATGGACCTCTGCGTTGATACCACTGCTTCCATGTACTCTGC10.15174506828528073Clontech Universal Primer Mix Long (95% over 23bp)
GGCACTAGAATGGACCTCTTCGCACTGCCTTGTTTTTAAGTGGCTAATGT10.15174506828528073No Hit
AAATACCGTTCAGAGCTGACTTGTTTTTAAGTGGCTAATGTATAACCATG10.15174506828528073No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA10.15174506828528073No Hit
GCACTAGAATGGACCTCTTCGCACTGCCTTGTTTTTAAGTGGCTAATGTA10.15174506828528073No Hit
GGCACTAGAATGGACCTCTTCCTAATCACTACATTATTTCAATTAAATAC10.15174506828528073No Hit
AAATACCGTTCAGAGCGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT10.15174506828528073No Hit
AAATACCGTTCGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT10.15174506828528073No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA10.15174506828528073No Hit
AAATACGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT10.15174506828528073No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACCTTATTTCAATTAAATAC10.15174506828528073No Hit
AAATACCGTTCAGAGCTGCCACTAGGAGCTTGCAAATTGCTGCTGGAACC10.15174506828528073No Hit
GCACTAGAATGGACCTCTTCGCAACTCTGCGTTGATAGCACTGCCTTGTT10.15174506828528073Clontech Universal Primer Mix Long (95% over 21bp)
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT10.15174506828528073No Hit
GGCACTAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACAA10.15174506828528073No Hit
GGCACTAGTGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACAA10.15174506828528073No Hit
AAATACCGTTCAGCACCGCCTTGTTTTAAGTGGCTAATGTAGAACCATGT10.15174506828528073No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA10.15174506828528073No Hit
GGCACTAGAATGGACCTCTTCTGCGTTGATAGCACTGCCTTGTTTTTAAG10.15174506828528073No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGAGTGCAAATTGCTGCTGGAAAC10.15174506828528073No Hit
CAATCCCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC10.15174506828528073No Hit
AAAACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT10.15174506828528073No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGTATAGCACT10.15174506828528073No Hit
GGCACTAGATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTGGC10.15174506828528073No Hit
AAATACCGTTCAGAGATGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC10.15174506828528073No Hit
GGCACTAGATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACAA10.15174506828528073No Hit
AAATACCGTTCAGAGCTCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT10.15174506828528073No Hit
AAATACCGTTCAGCACTGCCTGTTTTTAAGTGGCTAATGTAGAACCCATG10.15174506828528073No Hit
GGCACTAGAATGGACCTCTTCATCATCACTAAATTATTTCAATTAAATAC10.15174506828528073No Hit
AAATACAGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGATGGAACC10.15174506828528073No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA10.15174506828528073No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATATCAATTAAATAC10.15174506828528073No Hit
AAATACCGTTTAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG10.15174506828528073No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA10.15174506828528073No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGGGCTAATGTAGAACCATGT10.15174506828528073No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTCGGACC10.15174506828528073No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTGCTAATGTAGAAACATG10.15174506828528073No Hit
GGCACTAGAATGGACCTCTTCGCAACTCTGCGTTGATACCACTGCTTCCA10.15174506828528073Clontech Universal Primer Mix Long (96% over 25bp)
GGCACTAGAATGGACCTCTTCGCAACTCTGCGTTGATACCACTGCTTCCC10.15174506828528073Clontech Universal Primer Mix Long (96% over 26bp)
GGCACTAGAATGGACCTCTTCCTCATCACTACACTATTTCAATTAAATAC10.15174506828528073No Hit
AAATACCGTTCAGAGCTGCCTTATTTTTAAGTGGCTAATGTAGAACCATG10.15174506828528073No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAGTGGCTAATGTAGAACCATGT10.15174506828528073No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACA10.15174506828528073No Hit
AAATACCGTTCAAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT10.15174506828528073No Hit
GGCACTAGAATGGACCTCTCCGCAACTCTGCGTTGATAGCACTGCCTTGT10.15174506828528073Clontech Universal Primer Mix Long (95% over 21bp)
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA10.15174506828528073No Hit
AAATACCGTTCAGAGCTGCCTACGGTAGCACTGCCTTGTTTTTAAGTGGC10.15174506828528073No Hit
AAATACCTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT10.15174506828528073No Hit
GGCACTAGAATAGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC10.15174506828528073No Hit
AAATACCGGTCAGAGCTGCCTTGTTTTTAAGTGGCAAATGTAGAACCATG10.15174506828528073No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTATGGCTAATGTAGAACCATGTC10.15174506828528073No Hit
GGCACTAGAATGGATCTCTTCCTCATCACTCCATTATTTCAATTAAATAC10.15174506828528073No Hit
AAATACCTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT10.15174506828528073No Hit
AAATATCGTTCAGCGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGACCC10.15174506828528073No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGACCCATG10.15174506828528073No Hit
AAATACCGTTCAGAACTGCTTTGTTTTTAAGTGGCTAATGTAGAACCATG10.15174506828528073No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGGGCTAATGTAGAACCATGT10.15174506828528073No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAAGCATG10.15174506828528073No Hit
AAATACCGTTAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT10.15174506828528073No Hit
GGCACTAGAATGGACCTTTTCCTCATCACTACATTATTTCAATTAAATAC10.15174506828528073No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAGATTGCTGCTGGAACC10.15174506828528073No Hit
GGCACTAGAATGGACCTCTGCGTTGATACCACTGCCTTGTTTTTAAGTGG10.15174506828528073Clontech SMARTer II A Oligonucleotide (95% over 22bp)
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT10.15174506828528073No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGTCGGAACC10.15174506828528073No Hit
GGCATAGAATGGACCTCTTCGCAACTCTGCGTTGATAGCACTGCCTTGTT10.15174506828528073Clontech Universal Primer Mix Long (95% over 21bp)
AAATACCGTTAGAGCTGCCTTGTTTTAAGTGGCTAATGTAGAACCATGTC10.15174506828528073No Hit
AAATACGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT10.15174506828528073No Hit
AAATACCGTTCAAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT10.15174506828528073No Hit
AAATACCGTTCAGAGCTGCCTTGTTTGTAAGTGGCTAATGTAGAACCATG10.15174506828528073No Hit
AAATACCGTTCAGAGTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT10.15174506828528073No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGCACCATG10.15174506828528073No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACGTTATTTCAATTAAATAC10.15174506828528073No Hit
AAATAACGTTCAGAGCTGCCTTGTATTTAAGTGGCTAATGTAGAAACATG10.15174506828528073No Hit
GGACTAGAATGGACCTCTTCGCAACTCTGCGTTGATAGCACTGCCTTGTT10.15174506828528073Clontech Universal Primer Mix Long (95% over 21bp)
AAATACCGTTCAGAGCTGCCTCCAGGAGCTTGCAAATTCCTGCTGGAACC10.15174506828528073No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCATATTGCTGCTGGAACC10.15174506828528073No Hit
AAATACCGTTCAGAGCTGCCACCAGGGGCTTGCAAATTGCTGCTGGAACC10.15174506828528073No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCCAT10.15174506828528073No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTTAATAC10.15174506828528073No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACAATG10.15174506828528073No Hit
GGCACTAGAATGGACCTCTTCGTCATCACTACATTATTTCAATTAAATAC10.15174506828528073No Hit
AAATACCGTTCAAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT10.15174506828528073No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCACG10.15174506828528073No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAGTGGCTAATCTAGAACCAAGT10.15174506828528073No Hit
AATACCGGTCAGCACATCCGTTGTTTTAAGTTGCTAATGTAACACCATGT10.15174506828528073No Hit
AAATACCGTTCAGAGCTGCCACCGGGAGCTTGCAAATTGCTGCTGGAACC10.15174506828528073No Hit
AAATACCGTTCAGAACTGCCTTGGTTTTAAGTGGCTAATGTAGAACCATG10.15174506828528073No Hit
GGCACTAGAATGGACCTCTTCGTTGTTAGCACTGCCTTGTTTTTAAGGTG10.15174506828528073No Hit
AAATACCGTTCAGAACTGTCTTGTTTTTAAGTGGCTAATGTAGAACCATG10.15174506828528073No Hit
AAATACCGTTCAGCACTGCCTTTTTTTAAGTGGCTAATGTAGAACCATGT10.15174506828528073No Hit
GGCACTACAATGGACCTATTCATCATCAATACATTATTTAAATTAAATAC10.15174506828528073No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTACTGGAACC10.15174506828528073No Hit
TGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC10.15174506828528073No Hit
GGCACTAGAATGGACCTCTTCGCAACTCTGCGTTGATACCACTGCTTACA10.15174506828528073Clontech Universal Primer Mix Long (96% over 25bp)
AAATACCGTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT10.15174506828528073No Hit
GGCACTAGAATGGACCTCTTCGCAACTCTGCGTTGATACCACTGCTTACC10.15174506828528073Clontech Universal Primer Mix Long (96% over 26bp)
AAATACCGTTCAGAGCAGCCACCAGTAGCTTGCAAAATGATGATCGAACA10.15174506828528073No Hit
GCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACAA10.15174506828528073No Hit
AAATACGTTCAGAGCTGCCTTGTTTTTAGTGGCTAATGTAGAACCATGTC10.15174506828528073No Hit

[FAIL]Adapter Content

Adapter graph