FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152939

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152939
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1288
Total Bases186.7 kbp
Sequences flagged as poor quality0
Sequence length144-145
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCT45135.01552795031056No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTTGGACAGACCAAGTCTC27121.040372670807454No Hit
ACTTAAAAACAAGGCAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCTC21516.69254658385093No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTTTGGACAGACCAAGTCTC705.434782608695652No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG272.096273291925466No Hit
ACTTAAAAACAAGGCAGTGCTGAACGGTATTTTTGGACAGACCAAGTCTC251.9409937888198756No Hit
ACTTAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC191.4751552795031055No Hit
ACTTAAAAACAAGGCAGTGCTGTGCCCTCCCAGGTACCCCAAGCTGGCAG110.8540372670807453No Hit
ACTTAAAACAAGGCAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCTCT100.7763975155279503No Hit
TGTGTGTGTGTTTCTGTGGGTCCAGTTTGCTTGGCGAAGAGGTCCATTCT90.6987577639751553No Hit
TGTGTGTGTGTTTCTGTGGGTCCAGTTTGCTTGGCGTAGAGGTCCATTCT60.4658385093167702No Hit
ACTTAAAACAAGGCAGCTCTGAACGGTATTTTTGGACAGACCAAGTCTCT60.4658385093167702No Hit
ACTTAAAAACAAGGCAGTGCTATGCCCTCCCAGGTACCCCAAGCTGGCAG50.38819875776397517No Hit
ATTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC50.38819875776397517No Hit
ACTTAAAAACAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC40.3105590062111801No Hit
ACTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC40.3105590062111801No Hit
ACTTAAAAACAAGGCAGCGCTAATGCTAATGGTGCTACAGTTTCTGCCTC40.3105590062111801No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTGGACAGACCAAGTCTCT40.3105590062111801No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCC40.3105590062111801No Hit
ACTTAAAAACAAGGCAGCTCTGAACGTATTTTTGGACAGACCAAGTCTCT30.2329192546583851No Hit
ACTTAAAAACAGGCAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCTCT30.2329192546583851No Hit
ACTTAAAAACAAGGCAGTCTGAACGGTATTTTTGGACAGACCAAGTCTCT30.2329192546583851No Hit
ACTTAAAAACAAGGAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCTCT30.2329192546583851No Hit
ACTTAAAAACAAGGCACTCTGAACGGTATTTTTGGACAGACCAAGTCTCT30.2329192546583851No Hit
ACTTAAAAACAAGCAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCTCT20.15527950310559005No Hit
ACTTAAAAACAAGGCAGCTCTGACGGTATTTTTGGACAGACCAAGTCTCT20.15527950310559005No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCCGCCT20.15527950310559005No Hit
ACTTAAAAACAAGGCAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCCC20.15527950310559005No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAATTTCTGCCT20.15527950310559005No Hit
ACTTAAAAAAAGGCAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCTCT20.15527950310559005No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTTGGACAGACCAAGTCTCT20.15527950310559005No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTACTACAGTTTCTGCCT20.15527950310559005No Hit
ACTTAAAACAAGGCAGTTCTGAACGGTATTTTTGGACAGACCAAGTCTCT20.15527950310559005No Hit
ATTAAAAACAAGGCAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCTCT20.15527950310559005No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)