FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152940

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152940
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1288
Total Bases186.7 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC42633.07453416149068No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG26220.341614906832298No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC18914.673913043478262No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG705.434782608695652No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC251.9409937888198756No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG231.7857142857142856No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT141.0869565217391304No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT110.8540372670807453No Hit
GGCACTAGAATGGACCTCTTCGCCAAGCAAACTGGACCCACAGAAACACA80.6211180124223602No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT70.5434782608695652No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTGGCTAATGTAGAACCATGT70.5434782608695652No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT60.4658385093167702No Hit
GGCACTAGAATGGACCTCTACGCCAAGCAAACTGGACCCACAGAAACACA60.4658385093167702No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT40.3105590062111801No Hit
AAATACCGTTCAGAGTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT40.3105590062111801No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT40.3105590062111801No Hit
AAATACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT40.3105590062111801No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGATGCTGGAACC30.2329192546583851No Hit
AAATACCGTTAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT30.2329192546583851No Hit
AAATACGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT30.2329192546583851No Hit
AAATACCGTTCAGAGTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT30.2329192546583851No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT30.2329192546583851No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGACCC30.2329192546583851No Hit
AAATACCGTTCAGACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT30.2329192546583851No Hit
AAATACCGTTCAGAGTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT20.15527950310559005No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAACTGCTGCTGGAACC20.15527950310559005No Hit
AAATACCGTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT20.15527950310559005No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGTAAATTGCTGCTGGAACC20.15527950310559005No Hit
AAATACCGTTCAGAGCTGCCCCCAAGAGCTTGCAAATTGCTGCTGGAACC20.15527950310559005No Hit
GGCACTAGAATGGACCTCTACGCCAAGCAAACTGGGCCCACAGAAACACA20.15527950310559005No Hit
AAATACCGTTCAGAGCTCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT20.15527950310559005No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGATGCTGGAACC20.15527950310559005No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACA20.15527950310559005No Hit
AAATACCGTTCAGAGCTGCCCCCAGGAGCTTGCAAATTGCTGCTGGAACC20.15527950310559005No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGAAAATTGCTGCTGGAACC20.15527950310559005No Hit
AATACCTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCTG20.15527950310559005No Hit
AATACCGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT20.15527950310559005No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGGACC20.15527950310559005No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTGTCTAATGTAGAACCATG20.15527950310559005No Hit
AAATACCGTTCAGAGCGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT20.15527950310559005No Hit
AAATACCGTTCAGAGCTGCCACCAGGATCTTGCAAATTGCTGCTGGAACC20.15527950310559005No Hit
ATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCTG20.15527950310559005No Hit
AAATACCGTTCAGAGCTGCCTCCAGGAGCTTGCAAATTGCTGCTGGAACC20.15527950310559005No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCTTCCATGTACTCTGC20.15527950310559005Clontech SMARTer II A Oligonucleotide (95% over 22bp)
AAATACCGTTCAGAGCTGCCACCAGGGGCTTGCAAATTGCTGCTGGAACC20.15527950310559005No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGTTTGCAAATTGCTGCTGGAACC20.15527950310559005No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)