FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152941

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152941
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7486
Total Bases1 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG225830.162970878974082No Hit
ACTTAAAAACAAGGCAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCTC179824.018167245524978No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTTGGACAGACCAAGTCTC111314.86775313919316No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCT86811.594977290943094No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTTTGGACAGACCAAGTCTC3314.421586962329682No Hit
ACTTAAAAACAAGGCAGTGCTGAACGGTATTTTTGGACAGACCAAGTCTC630.84157093240716No Hit
ACTTAAAACAAGGCAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCTCT520.6946299759551162No Hit
ACTTAAAACAAGGCAGCTCTGAACGGTATTTTTGGACAGACCAAGTCTCT260.3473149879775581No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTGGACAGACCAAGTCTCT250.33395671920919046No Hit
ACTTAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC210.28052364413572No Hit
ACTAAAAACAAGGCAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCTCT160.21373230029388193No Hit
TGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGCT140.18701576275714668No Hit
ACTTAAAAACAGGCAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCTCT140.18701576275714668No Hit
ACTTAAAAACAAGGCAGCTCTGAACGTATTTTTGGACAGACCAAGTCTCT130.17365749398877905No Hit
TGTGTGTGTGTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC130.17365749398877905No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAGG110.1469409564520438No Hit
ACTTAAAAACAAGGCAGTGCTATCAACGAAGAGGTCCATTCTAGTGCCTT110.1469409564520438No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGGCAGAAGGGTAAAG110.1469409564520438No Hit
ACTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC90.12022441891530856No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTTGGACAGACCAAGTCTCT80.10686615014694097No Hit
ACTTAAAAACAAGGCAGTGCTAATGCTAATGGTGCTACAGTCTCTGCCTC80.10686615014694097No Hit
ACTTAAAAACAAGGCAGTGCTGTGCCCTCCCAGGTACCCCAAGCTGGCAG80.10686615014694097No Hit
ACTTAAAAACAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC80.10686615014694097No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)