FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152954

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152954
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4938
Total Bases715.8 kbp
Sequences flagged as poor quality0
Sequence length141-145
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC127525.820170109356017No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC91218.469015795868774No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG73414.864317537464562No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC4238.566221142162819No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC3386.844876468205752No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG1653.341433778857837No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG460.9315512353179425No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT400.8100445524503848No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA240.48602673147023084No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT240.48602673147023084No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT220.44552450384771164No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA180.3645200486026731No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT180.3645200486026731No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTGGCTAATGTAGAACCATGT130.26326447954637505No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTGG110.22276225192385582No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA110.22276225192385582No Hit
AAATACGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT110.22276225192385582No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA110.22276225192385582No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA110.22276225192385582No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA100.2025111381125962No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT100.2025111381125962No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA100.2025111381125962No Hit
AAATACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT90.18226002430133656No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA90.18226002430133656No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA80.16200891049007696No Hit
AAATACCGTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT80.16200891049007696No Hit
GGCACTAGAATGGACCCTTCCTCATCACTACATTATTTCAATTAAATACA70.14175779667881733No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA70.14175779667881733No Hit
AATACCGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT60.12150668286755771No Hit
AAATACCGTTCAGACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT60.12150668286755771No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA60.12150668286755771No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACA60.12150668286755771No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCGAATTGCTGCTGGAACC60.12150668286755771No Hit
AAATACCGTTCGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT60.12150668286755771No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAGTGGCTAATGTAGAACCATGT60.12150668286755771No Hit
GGCACTAGAATGGACCTCTTCCTCACCACTACATTATTTCAATTAAATAC50.1012555690562981No Hit
AAATACCGTTCAGAGTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT50.1012555690562981No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA50.1012555690562981No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTACATTATTTCAATTAAATAC50.1012555690562981No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAGTAC50.1012555690562981No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA50.1012555690562981No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)