FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152964

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152964
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1585
Total Bases229.7 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC64340.56782334384858No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG25516.08832807570978No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC21313.438485804416404No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG825.173501577287067No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG231.451104100946372No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA201.2618296529968454No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC140.8832807570977917No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA130.8201892744479496No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA110.694006309148265No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT100.6309148264984227No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT90.5678233438485805No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT70.4416403785488959No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA60.3785488958990536No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA60.3785488958990536No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA60.3785488958990536No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT50.31545741324921134No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTGG40.2523659305993691No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA40.2523659305993691No Hit
AAATACCGTTCAGACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT40.2523659305993691No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA40.2523659305993691No Hit
AAATACCGTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT30.1892744479495268No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCCTGCAAATTGCTGCTGGAACC30.1892744479495268No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA30.1892744479495268No Hit
GGCACTAGAATGGACCCTTCCTCATCACTACATTATTTCAATTAAATACA30.1892744479495268No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA30.1892744479495268No Hit
AAATACCTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT30.1892744479495268No Hit
GGCACTAGAATGGCCTCTTCCTCATCACTACATTATTTCAATTAAATACA30.1892744479495268No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTGGCTAATGTAGAACCATGT30.1892744479495268No Hit
GGCACTAGAATGGACCTCTTCGTCATCACTACATTATTTCAATTAAATAC30.1892744479495268No Hit
GGCACTAGAATGGACCTCTCCCTCATCACTACATTATTTCAATTAAATAC20.12618296529968456No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAATGGCTAATGTAGAACCATGT20.12618296529968456No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAAATAAATAC20.12618296529968456No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACAGGCTAATGTAGAA20.12618296529968456No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA20.12618296529968456No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT20.12618296529968456No Hit
GACACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC20.12618296529968456No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACA20.12618296529968456No Hit
AAATACCGTTAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT20.12618296529968456No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTCCAATTAAATAC20.12618296529968456No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACGTTATTTCAATTAAATAC20.12618296529968456No Hit
GGCACTAGAATGGACCTCTTCCTCATAACTACATTATTTCAATTAAATAC20.12618296529968456No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAACAC20.12618296529968456No Hit
AAATACCGTTCAGAGCTGCCACCGGGAGCTTGCAAATTGCTGCTGGAACC20.12618296529968456No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTACTGGAACC20.12618296529968456No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCTCCCATGTACTCTGC20.12618296529968456Clontech SMARTer II A Oligonucleotide (95% over 21bp)
AAATACCTTCAGCACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT20.12618296529968456No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT20.12618296529968456No Hit
GGCACTAGAATGGACCTCGTCCTCATCACTACATTATTTCAATTAAATAC20.12618296529968456No Hit
GGAACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC20.12618296529968456No Hit
GGCACTAGAATGGACCTCTTCCCCATCACTACATTATTTCAATTAAATAC20.12618296529968456No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCTTCCATGTACTCTGC20.12618296529968456Clontech SMARTer II A Oligonucleotide (95% over 22bp)
GGCACTAGAATGGACCTTTTCCTCATCACTACATTATTTCAATTAAATAC20.12618296529968456No Hit
GGCACTAGAATGGACCTCCTCCTCATCACTACATTATTTCAATTAAATAC20.12618296529968456No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)