FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152969

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152969
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3874
Total Bases561.6 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCT137635.51884357253485No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG121131.259679917398035No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTTGGACAGACCAAGTCTC52713.603510583376355No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTTTGGACAGACCAAGTCTC1704.388229220443986No Hit
ACTTAAAAACAAGGCAGTGCTGAACGGTATTTTTGGACAGACCAAGTCTC391.006711409395973No Hit
ACTTAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC340.8776458440887971No Hit
ACTTAAAAACAAGGCAGTGCTGTGCCCTCCCAGGTACCCCAAGCTGGCAG170.43882292204439854No Hit
ACTTAAAAACAAGGCAGTGCTATCAACGAAGAGGTCCATTCTAGTGCCTT140.3613835828600929No Hit
ACTTAAAAACAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC110.2839442436757873No Hit
TGTGTGTGTGTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC110.2839442436757873No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTGGACAGACCAAGTCTCT100.2581311306143521No Hit
ACTTAAAAACAAGGCAGTGCTATGCCCTCCCAGGTACCCCAAGCTGGCAG90.23231801755291687No Hit
ACTTAAAACAAGGCAGCTCTGAACGGTATTTTTGGACAGACCAAGTCTCT80.20650490449148168No Hit
ACTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC80.20650490449148168No Hit
ACTTAAAAACAAGGCAGCTCTGACGGTATTTTTGGACAGACCAAGTCTCT70.18069179143004646No Hit
ACTTAAAAACAAGGCAGCTCTGAACGTATTTTTGGACAGACCAAGTCTCT60.15487867836861124No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGGCAGAAGGGTAAAG60.15487867836861124No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTGAAG50.12906556530717606No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTTGGACAGACAAAGTCTC50.12906556530717606No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCCGCCT50.12906556530717606No Hit
ACTTAAAAACAAGGCGGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCT50.12906556530717606No Hit
ACTTAAAAACAAGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC40.10325245224574084No Hit
TGTGTGTGCGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG40.10325245224574084No Hit
CGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG40.10325245224574084No Hit
ACTTAAAAACAAGGCAGTCTGAACGGTATTTTTGGACAGACCAAGTCTCT40.10325245224574084No Hit
TGTGTGTGTGTTTTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC40.10325245224574084No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAGGGGTAAAG40.10325245224574084No Hit
ACTTAAAACCAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCT40.10325245224574084No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGTCT40.10325245224574084No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTCTGGACAGAAGGGTAAAG40.10325245224574084No Hit
ACTTAAAAAAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC40.10325245224574084No Hit
ACTTAAAACAAGGCAGTTCTGAACGGTATTTTTGGACAGACCAAGTCTCT40.10325245224574084No Hit
ATTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC40.10325245224574084No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAGGGTTTGGACAGAAGGGTAAAG40.10325245224574084No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)