FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152970

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152970
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3874
Total Bases561.5 kbp
Sequences flagged as poor quality0
Sequence length141-145
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC66817.24315952503872No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC55814.403717088280846No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC54614.093959731543624No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG52013.422818791946309No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC50613.061435209086216No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG1634.207537429013939No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG370.9550851832731028No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT200.5162622612287042No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT170.43882292204439854No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTGG130.33557046979865773No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA130.33557046979865773No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT130.33557046979865773No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA100.2581311306143521No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT90.23231801755291687No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTGGCTAATGTAGAACCATGT80.20650490449148168No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA80.20650490449148168No Hit
AAATACCGTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT80.20650490449148168No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT80.20650490449148168No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA70.18069179143004646No Hit
AAATACCGTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT70.18069179143004646No Hit
AAATACGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT60.15487867836861124No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA60.15487867836861124No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA60.15487867836861124No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA60.15487867836861124No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGATTGCAAATTGCTGCTGGAACC60.15487867836861124No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA50.12906556530717606No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAAAAC50.12906556530717606No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA50.12906556530717606No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCCGGAACC50.12906556530717606No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGATGCTGGAACC50.12906556530717606No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGATGCTGGAACC50.12906556530717606No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA50.12906556530717606No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA50.12906556530717606No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT40.10325245224574084No Hit
AAATACCGTTCAGAGCAGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC40.10325245224574084No Hit
AAATACCGTTCAGAGCTGCCAACAGGAGCTTGCAAATTGCTGCTGGAACC40.10325245224574084No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGACCC40.10325245224574084No Hit
AAATACCGTTCAGACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT40.10325245224574084No Hit
AAATACCGTTCGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT40.10325245224574084No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA40.10325245224574084No Hit
AAATACCGTTAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT40.10325245224574084No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGTTGGAACC40.10325245224574084No Hit
AAATACCGTTCAGAACTGCCTTGTTTTAAGTGGCTAATGTAGAACCATGT40.10325245224574084No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCCTGCAAATTGCTGCTGGAACC40.10325245224574084No Hit
GGCACTAGAATGGACCTCTCCCTCATCACTAAATTATTTCAATTAAATAC40.10325245224574084No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA40.10325245224574084No Hit
AAATACCGTTCAGAGCTGACACCAAGAGCTTGCAAATTGCTGCTGGAACC40.10325245224574084No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTAAATTAAATAC40.10325245224574084No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGACCC40.10325245224574084No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)