FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152979

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152979
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9807
Total Bases1.4 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG326733.31293973692261No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCT239924.462118894667075No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTTGGACAGACCAAGTCTC205820.985010706638114No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTTTGGACAGACCAAGTCTC4684.7721015601101255No Hit
ACTTAAAAACAAGGCAGTGCTGTGCCCTCCCAGGTACCCCAAGCTGGCAG1351.3765677577240747No Hit
ACTTAAAAACAAGGCAGTGCTGAACGGTATTTTTGGACAGACCAAGTCTC850.8667278474558988No Hit
ACTTAAAACAAGGCAGCTCTGAACGGTATTTTTGGACAGACCAAGTCTCT530.5404303048842664No Hit
ACTTAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC510.5200367084735393No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTGGACAGACCAAGTCTCT490.4996431120628123No Hit
ACTTAAAAACAAGGCAGTGCTATGCCCTCCCAGGTACCCCAAGCTGGCAG410.41806872641990417No Hit
ACTTAAAAACAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC250.2549199551340879No Hit
ACTTAAAAACAAGGCAGCTCTGAACGTATTTTTGGACAGACCAAGTCTCT210.21413276231263384No Hit
ACTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC200.20393596410727033No Hit
ACTTAAAAACAAGGCAGTCTGAACGGTATTTTTGGACAGACCAAGTCTCT190.1937391659019068No Hit
TGTGTGTGTGTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC170.17334556949117977No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAGG160.16314877128581626No Hit
ACTTAAAAACAAGGCAGTGCTATCAACGAAGAGGTCCATTCTAGTGCCTT160.16314877128581626No Hit
ACTTAAAAACAAGGCAGCTTGAACGGTATTTTTGGACAGACCAAGTCTCT150.15295197308045275No Hit
ACTTAAAAACAAGGCAGCTCTGACGGTATTTTTGGACAGACCAAGTCTCT140.14275517487508924No Hit
ACTTAAAAAAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC140.14275517487508924No Hit
CCTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCT130.1325583766697257No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGGAGGGTAAAG110.11216478025899868No Hit
TGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGCT110.11216478025899868No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAGGGTTTGGACAGAAGGGTAAAG110.11216478025899868No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTTGGACAGACAAAGTCTC110.11216478025899868No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTTGGACAGACCAAGTCTCT100.10196798205363516No Hit
ACTTAAAAACAAGGCAGCGCTAATGCCTAATGGTGCTACAGTTTCTGCCT100.10196798205363516No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGGCAGAAGGGTAAAG100.10196798205363516No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)