FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152984

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152984
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences704
Total Bases102 kbp
Sequences flagged as poor quality0
Sequence length144-145
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC26838.06818181818182No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG11416.193181818181817No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC7911.221590909090908No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC618.664772727272728No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG253.5511363636363638No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT70.9943181818181818No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT40.5681818181818182No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT40.5681818181818182No Hit
AAATACCGTTCAGAGTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT40.5681818181818182No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA40.5681818181818182No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG40.5681818181818182No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA40.5681818181818182No Hit
AAATACCGTTCAAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT30.4261363636363636No Hit
AAATACCGTTCGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT20.2840909090909091No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAAATAAATAC20.2840909090909091No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA20.2840909090909091No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA20.2840909090909091No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAAAC20.2840909090909091No Hit
AAAACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT20.2840909090909091No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAGTGGCTAATGTAGAACCATGT20.2840909090909091No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA20.2840909090909091No Hit
AAATACCGTTAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT20.2840909090909091No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATCGCTGCTGGAACC20.2840909090909091No Hit
AAATACCGTTAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT20.2840909090909091No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGATTGCAAATTGCTGCTGGAACC20.2840909090909091No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTGGCTAATGTAGAACCATGT20.2840909090909091No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT20.2840909090909091No Hit
AAATACCGTTCAGGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT20.2840909090909091No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA10.14204545454545456No Hit
AAATACCGTACAGAGATCCCACAAGACCATTCAAAATTGATGATGGAACA10.14204545454545456No Hit
AAATACCGTTAGCACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT10.14204545454545456No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGACCC10.14204545454545456No Hit
AAATACCGTTCGGCACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG10.14204545454545456No Hit
AAAAACCGATCAGAGCTGCCACCACGAGCTTCCAAATAGCTCCTCGAACC10.14204545454545456No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTACAATTAAATAC10.14204545454545456No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTGCTAATGTAGAACAATG10.14204545454545456No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAAATAAATAC10.14204545454545456No Hit
AAAAACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTCGAACC10.14204545454545456No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGATTGCAAATTTCTGCTGGAACC10.14204545454545456No Hit
GGCACTAGAATGAACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC10.14204545454545456No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC10.14204545454545456No Hit
AAATCCCGTTCAGAGCTGCCACCAGGCGCTTGCAAATTGCTGATGAAACC10.14204545454545456No Hit
GGTACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC10.14204545454545456No Hit
AAATACCGTTCAGAGCTGTCAGCTTGGGGTACCTGGGAGGGCATAGCACT10.14204545454545456No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAGTTGCTGCTGGAACC10.14204545454545456No Hit
AAATACCGTTCAGACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT10.14204545454545456No Hit
GGCACTAGAATGGACCTCTGCGTTGATACCACTGCTTCCATGTACTCTGC10.14204545454545456Clontech Universal Primer Mix Long (95% over 23bp)
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGAAACC10.14204545454545456No Hit
AAATACAGTTCAGAGCTGCCACCAGGAGCTTGCAAATAGCTGCTGGAATC10.14204545454545456No Hit
AAATACCGTTCAGAGCGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT10.14204545454545456No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA10.14204545454545456No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATCTCAATTAAATAC10.14204545454545456No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGATGCTGGAACC10.14204545454545456No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTAAATTAAATAC10.14204545454545456No Hit
AAAAACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC10.14204545454545456No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGATTGCACAGCACT10.14204545454545456No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA10.14204545454545456No Hit
GGCACTAGATTGGACCCTTCCTCATCACTAAATTATTTCAATTAAATACA10.14204545454545456No Hit
AAATACCGTTCTGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC10.14204545454545456No Hit
AAATACCGTTCAGAGCTGCCACCAGGCGCTTGCAAATTGCTGCTGGAACC10.14204545454545456No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGACCATGT10.14204545454545456No Hit
AAATACCGTTCAGAGCTGCCGCCAGGAGCTTGCAAATTGCTGCTGGAACC10.14204545454545456No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA10.14204545454545456No Hit
AAATACCGTTCAGAGCTGCCTGCTTTAGAATGCTATAAGCTACAGGCAGC10.14204545454545456No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA10.14204545454545456No Hit
AAACACACACACAGGCTAATGTAGAACCATGTCGTCAGTGTAGATCTCGG10.14204545454545456No Hit
AAAAAACGTTAAGAGCTGCTACCAGGAGATTGCAAATAGCTGCTGGAACA10.14204545454545456No Hit
AAATACCGATCAGAGCTGCCACCAAGAGCATGAAAATTGCTGCTGGAACC10.14204545454545456No Hit
AAATACCCTTCAGAGCTTCCTTGTTTTTAAGTGGCTAATGTAGAACCATG10.14204545454545456No Hit
GGCACTAGAATGGACATCTTCCTCATCACTAAATTATTTCAATTAAAAAC10.14204545454545456No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA10.14204545454545456No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTTGAAAC10.14204545454545456No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACG10.14204545454545456No Hit
AAATACCGTTCAGAGCTGACACCAGGAGCTTGCAAATTGCTGCTGGAACC10.14204545454545456No Hit
AAATACCGTGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCTGA10.14204545454545456No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTCCAAAAAGCAGCTGGAACC10.14204545454545456No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGTTGCTGGAACC10.14204545454545456No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA10.14204545454545456No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATAGCAGCTGGAACC10.14204545454545456No Hit
GGTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACAAT10.14204545454545456No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTGG10.14204545454545456No Hit
AAAACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT10.14204545454545456No Hit
AAATACCATTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC10.14204545454545456No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACA10.14204545454545456No Hit
AAATACCGTTCAGAGATGCCACCAGGAGATTGCAAATTGCTGCTGGAACC10.14204545454545456No Hit
GGCACTAGAATGGACCCTTCCTCATCACTACATTATTTCAATTAAATACA10.14204545454545456No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGATGGAACC10.14204545454545456No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGGTGGAACC10.14204545454545456No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAATC10.14204545454545456No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTACTGCTGGAACC10.14204545454545456No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGACCCT10.14204545454545456No Hit
AAATACCGTTCAGAGCTGCCCCCAGGAGCTTGCAAATTGCTGCTGGAACC10.14204545454545456No Hit
AAATACCGTTCACCGCTCCCACCAGGAGCTAGCAAATAGCTGCTGGAACC10.14204545454545456No Hit
AAACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGTC10.14204545454545456No Hit
AAATACCGTTCAGAGCTGCTACCAGGAGCTTGCAAATTGCTGCTGGAACC10.14204545454545456No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGAAAATTGCTGCTGGAACC10.14204545454545456No Hit
AATACCGTTCAGAGCTGCCATCAGGAGCTTGCAAATTGCTGCTGGAACCT10.14204545454545456No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAAAAC10.14204545454545456No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTTCAAATTGCTGCTTGAACC10.14204545454545456No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT10.14204545454545456No Hit
AAATACCGTTCAGAACTGCCTTGTTATTAAGTGGCTAATGTAGAACCATG10.14204545454545456No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAAGTGCTGCTGGAACC10.14204545454545456No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA10.14204545454545456No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACGTTATTTCAATTAAATAC10.14204545454545456No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT10.14204545454545456No Hit
AAATACCGTTCAGCGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG10.14204545454545456No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATTTAGAACCATG10.14204545454545456No Hit
AAATACCGTTCAGAGCTGCCACCAGGGGCTTGCAAATTGCTGCTGGAACC10.14204545454545456No Hit
AAATACCGTTCAGAGCTGCCACAAGGAGATTGCAAATTGCTGCTGGAACC10.14204545454545456No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAGTGGCTAATGTAGAACCATGT10.14204545454545456No Hit
AAATACCGTTCAGAGCTGCCACCGGGAGCTTGCAAATTGCTGCTGGAACC10.14204545454545456No Hit
GGCACTAGAATGGACTTCTTCCTCATCACTACATTATTTCAATTAAATAC10.14204545454545456No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTACTGGAACC10.14204545454545456No Hit
AAATACCGTTCACAGCAACCACCAGGAGCTACCAAATAGCACCTGCAACC10.14204545454545456No Hit
TGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC10.14204545454545456No Hit
AAATACCGTTCAGAGCTGCCACCAGTAGCTTGCAAATTGCTGCTGGAACC10.14204545454545456No Hit
AAATAACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTCCTGCTGGAACC10.14204545454545456No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATAGCTGCTGGAACCT10.14204545454545456No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTATATTATTTCAATTAAATAC10.14204545454545456No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTGAATAC10.14204545454545456No Hit
CGCACAAGAATGGACCTCATCCTCATCACTAAATTATTTCAAATAAATAC10.14204545454545456No Hit

[FAIL]Adapter Content

Adapter graph