FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152988

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152988
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences847
Total Bases122.8 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC20223.84887839433294No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG16619.59858323494687No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC10612.514757969303425No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC839.799291617473434No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC586.8476977567886665No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG424.958677685950414No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG91.062573789846517No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT70.8264462809917356No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT50.5903187721369539No Hit
AAATACCGTTAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT30.3541912632821724No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT30.3541912632821724No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTGGCTAATGTAGAACCATGT30.3541912632821724No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT30.3541912632821724No Hit
GGCCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATA20.23612750885478156No Hit
AAATACCGTTCGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT20.23612750885478156No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCCTGCAAATTGCTGCTGGAACC20.23612750885478156No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT20.23612750885478156No Hit
AAATACCGTTCAGGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT20.23612750885478156No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA20.23612750885478156No Hit
AAATACCGTTTAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC20.23612750885478156No Hit
AAATACCGTTCAGAACTGCCTTGTTTTAAGTGGCTAATGTAGAACCATGT20.23612750885478156No Hit
AAATACCGTTCAGAGCTGCCCCCAGGAGCTTGCAAATTGCTGCTGGAACC20.23612750885478156No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATT20.23612750885478156No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATAGCTGCTGGAACC20.23612750885478156No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA20.23612750885478156No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGGACC20.23612750885478156No Hit
GGCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACAA10.11806375442739078No Hit
AAATACCGTTCAGAGTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT10.11806375442739078No Hit
AAATACGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGACCCATGT10.11806375442739078No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCAAGT10.11806375442739078No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAACTGCTGCTGGAACC10.11806375442739078No Hit
AAATACCGTTCAGAGCTGCCACCATGAGCTTGCAAATTGCTGCCGGAACC10.11806375442739078No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAACTATTTCAATTAAATAC10.11806375442739078No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA10.11806375442739078No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGAAAATTGATGCTGGAACC10.11806375442739078No Hit
AAATACCGTTCAGAGCTGCCACCATGAGCTTGCAAATTGCTGCTGGAACC10.11806375442739078No Hit
AAATACCGTTCAAGCTGCCACCAGGAGCTTGCAAATTGCTCCTGGAACCT10.11806375442739078No Hit
AAATCCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT10.11806375442739078No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCCTTGTTTTTAAGTGG10.11806375442739078No Hit
GGCACAAGAATGGACCTCTTCCACATCACCACAATATATCAATTAAATAC10.11806375442739078No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTGGATAATGTAGAACCATG10.11806375442739078No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTATGTGGCTAATGTAGAACCATG10.11806375442739078No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAAGTAAATAC10.11806375442739078No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCATGCAAATTGCTGCTGGAACCT10.11806375442739078No Hit
GGCACTAGATTGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC10.11806375442739078No Hit
AAATACCGTTCAGAGCTGTCTTGTTTTTAAGTGGCTAATGTAGAACCATG10.11806375442739078No Hit
AAAAACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC10.11806375442739078No Hit
GGTACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA10.11806375442739078No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGAGGCTAATGTAGAACCATG10.11806375442739078No Hit
AAATACCGTTCAGAGCGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT10.11806375442739078No Hit
AAATACCGTTCAGAGCTGCAACCAGGAGCTTGCAAATTGCTGCTGGAACC10.11806375442739078No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAGTTGCTGCTGGAACG10.11806375442739078No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGATGGAACC10.11806375442739078No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTTAATAC10.11806375442739078No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTCGAACC10.11806375442739078No Hit
AAATACCATTCACATAAGCAACAAAGAGCTTGCAAATTGCTGCTGGAACC10.11806375442739078No Hit
AAATAACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC10.11806375442739078No Hit
GGCACTAGAATGGACCTCTGCCTCCGCACTAAATTATTTCAATTAAATAC10.11806375442739078No Hit
AAATACCGTTCAGAGCTCCCACCAAGAGCTTGCAAATTGCTGCTGGAACC10.11806375442739078No Hit
ATACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCTGA10.11806375442739078No Hit
AAATACCGTTCAGAGCTGCCAACAGGGTAGGCCCGCGGGCCACAGAGTGA10.11806375442739078No Hit
AAATACCGTTCAGAGATGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG10.11806375442739078No Hit
ATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGTC10.11806375442739078No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTCCTGCTGGAACC10.11806375442739078No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAGATTATTTCAATTAAATAC10.11806375442739078No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAAAC10.11806375442739078No Hit
GGCACTAGAAGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACAA10.11806375442739078No Hit
AAATACCGTTCCGGGCTGCCACCAGGCCCTTGCAAATAGCTGCTGGAACC10.11806375442739078No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGGCCC10.11806375442739078No Hit
AAAACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT10.11806375442739078No Hit
AAATACCGTTCAGAGCTGCCGCCAGGAGCTTGCAAATTGCTGCTGGAACC10.11806375442739078No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA10.11806375442739078No Hit
AAATACCGTTCAGAACTGCCATGTTTTTAAGTGGCTAATGTAGAACCATG10.11806375442739078No Hit
AAATACCGTTTAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC10.11806375442739078No Hit
AAATACCGTTCGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT10.11806375442739078No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAGCCT10.11806375442739078No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATGC10.11806375442739078No Hit
AAAAACCGTTCAGAGATGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC10.11806375442739078No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA10.11806375442739078No Hit
GGCACTAGAATGGACCTCGTCCTCATCACAAAATTAATACAAATAAATAC10.11806375442739078No Hit
AAATAACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC10.11806375442739078No Hit
AAATACCGTTCAGAGCTGCCACCAAGACCTACCAAATTGCAGCTGGAACC10.11806375442739078No Hit
AAATACCGTTCAGAGCTGCCACCAGAAGCTTGCAAATTGCTGCTGGAACC10.11806375442739078No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGATGCTGGAACC10.11806375442739078No Hit
AAATGCCGTTCAGAGCAGCCACCAGCACCTTCCAAATAGACGCTGCAACC10.11806375442739078No Hit
AAATACCGTTAAGAGCTGACTTGTTTTTAAGTGGCTAATGTAGAACCATG10.11806375442739078No Hit
AAATACTGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC10.11806375442739078No Hit
AAATACCGTTAAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAAACATG10.11806375442739078No Hit
AAATACCGTTCAGAGAGCCACCAAGAGCTTCCATATTGATCCTTGAACCT10.11806375442739078No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGATTGCAAATTGCTGCTGGAACC10.11806375442739078No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTGG10.11806375442739078No Hit
AATACCGTTCAGGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCTG10.11806375442739078No Hit
AAATACCGTTCAGAGTTGCCACCAGGAGCTTGCAAATTGATGCTGGAACC10.11806375442739078No Hit
AAATACCGTTCAGAGCTGCCTACGGTATCACTCCCTTGTTATTAAGTGGC10.11806375442739078No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACACTATTTCAATTAAATAC10.11806375442739078No Hit
AAATACCGTTCAGAGCTACCACCAGGAGCTTGCAAATTGCTGCTGGAACC10.11806375442739078No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGATTGCAAATTGCAGCTGGAACC10.11806375442739078No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAGTGGCTAATGTAGAACCATGT10.11806375442739078No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA10.11806375442739078No Hit
AAATACCGTTCAGAGCTGCCACCAAGCGCTTGCAAATTGCTGCTGGAACC10.11806375442739078No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGTTGGAACC10.11806375442739078No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACA10.11806375442739078No Hit
AAAAACCGTTAAGAGCTGAAACAAGTAGATTGCAAATTGCTGCTGGAACC10.11806375442739078No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTTGAACCATG10.11806375442739078No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGATGGAACC10.11806375442739078No Hit
GGCATAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACAA10.11806375442739078No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA10.11806375442739078No Hit
AAATCCCGTAAGCGCGTCCCCCAAGTGCCTTGAAATTTCATCATGGAACG10.11806375442739078No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAAGACA10.11806375442739078No Hit
AAATATCGTTCAGAGCTGCCACCAAGAGTTTGCAAATTGCTGCTGGAACC10.11806375442739078No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTAAATTATTTCAATTAAATAC10.11806375442739078No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAACTGGCTAATGTAGAACCATG10.11806375442739078No Hit
AAATACCGTTCACAGCAGCCACCAGGAGCTTGCAAATTGCAGCTGGAACC10.11806375442739078No Hit
AAGTACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGTTGCTTGAAAC10.11806375442739078No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGAAAATTGCTGCTGGAACA10.11806375442739078No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCATGCAAATTGCTGCTGGAACC10.11806375442739078No Hit
AAATACCGTTCACAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC10.11806375442739078No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCTTTGTTTTTAAGTGG10.11806375442739078Clontech SMARTer II A Oligonucleotide (95% over 22bp)
AAATACCTTAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCTG10.11806375442739078No Hit
AAATACCGTTCAGAGCGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT10.11806375442739078No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA10.11806375442739078No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTACAAATTGCTGCTGGAACC10.11806375442739078No Hit
AAATACCGATCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC10.11806375442739078No Hit
AAATACGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT10.11806375442739078No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGACCC10.11806375442739078No Hit
AAATACCGTTCAGAGTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT10.11806375442739078No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTCCAATTAAATAC10.11806375442739078No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTGAAGTGGCTAATGTAGAACCATG10.11806375442739078No Hit
AAATACCGTTCAGAGCTGCCCTTGTTTTTAAGTGGCTAATGTAGAACCAT10.11806375442739078No Hit
AAATACCGTTCAGAGCGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT10.11806375442739078No Hit
AAATACCGTTCAGAGCTCCCACCAGGACCTTGCAAATAGCTGCTGGAACC10.11806375442739078No Hit
AAATACCGTTCAAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT10.11806375442739078No Hit
AAATACCGTTAAGAGCAGCCACCAGGAGACTGCAAAATGATACAGGAACC10.11806375442739078No Hit
AAATACCGTTCAGAGCTGCCACTAAGAGCTTGCAAATTGCTGCTGGAACC10.11806375442739078No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT10.11806375442739078No Hit
AAATACCGTTCAGAGCTGCCACAAGGAGCTTGCAAATTGCTACTGTAACC10.11806375442739078No Hit
AAATACCGTTCAGAGCTGCCACCTGGAGCTTGCAAATTGCTGCTGGAACC10.11806375442739078No Hit
AAATACCGTTCAGAGCTGCCACCAGGGGCTTGCAAATTGCTGCTGGAACC10.11806375442739078No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACAATG10.11806375442739078No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTCCAAATTGCTGCTGGAACC10.11806375442739078No Hit
AAATACCGTTCAGAGCTGCCTGTTTTTACGTGGCTAATGTAGAACCATGT10.11806375442739078No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCACG10.11806375442739078No Hit
AAATACCGTTCAGAGCTTCCACCAAGAGCTTTCAAATTGCTGCTGGAAAC10.11806375442739078No Hit
AAATACCGTTCAGGACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG10.11806375442739078No Hit
AAATCCAGTTAAGAGCTGCCACCAAGAGATTGCAAATTTCTGCTGGAACC10.11806375442739078No Hit
AAATACCGGTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG10.11806375442739078No Hit
AAATACCGTTCAGAGCTGCCACCAGTAGCTTGCAAATTGCTGCTGGAACC10.11806375442739078No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGAGGCTGGAACA10.11806375442739078No Hit
AAATACCGTTCAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAGCCTG10.11806375442739078No Hit
AAATAACGTTCACAGCTGCCCCCAGCAGATTGCAAATTGAAGATTTAACC10.11806375442739078No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTAAAGTAAATAC10.11806375442739078No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTTCAAATTGCTGCTGGAACC10.11806375442739078No Hit
AAATACCGTTCAGAGCTGACACCAGGAGCTTGCAAATTTATGCTGGAACC10.11806375442739078No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTCGCAAATTGCTGCTGGACCC10.11806375442739078No Hit
AAATACCGTTCAGAGCTGCCACACACACACCCACAGAAACACACACACAG10.11806375442739078No Hit
GGCACTAGAATGGGCCTCTTCCTCATCACTAAATTATTTCAATTAAATAC10.11806375442739078No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTGAATAC10.11806375442739078No Hit

[FAIL]Adapter Content

Adapter graph