FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152990

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152990
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4951
Total Bases717.6 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC120024.23752777216724No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC70814.300141385578671No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG67813.69420319127449No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC54110.927085437285397No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC53110.725106039184002No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG1603.2316703696222984No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT340.6867299535447384No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG310.6261361341143203No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTGG210.42415673601292664No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA190.38376085639264795No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA150.30296909715209047No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTGGCTAATGTAGAACCATGT150.30296909715209047No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT150.30296909715209047No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA140.28277115734195113No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA140.28277115734195113No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT130.2625732175318118No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA110.22217733791153302No Hit
AAATACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT110.22217733791153302No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA100.20197939810139365No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA100.20197939810139365No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA100.20197939810139365No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT90.1817814582912543No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT90.1817814582912543No Hit
GGCACTAGAATGGACCTCTTCGTCATCACTAAATTATTTCAATTAAATAC80.1615835184811149No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA80.1615835184811149No Hit
AAATACCGTTAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT80.1615835184811149No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA80.1615835184811149No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA70.14138557867097556No Hit
AAATACCGTTAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT70.14138557867097556No Hit
AAATACCGTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT70.14138557867097556No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA70.14138557867097556No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTAAATTAAATAC70.14138557867097556No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA60.12118763886083621No Hit
GGCACTAGAATGGACCCTTCCTCATCACTAAATTATTTCAATTAAATACA60.12118763886083621No Hit
AAATACCGTTCAGAGTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT60.12118763886083621No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA60.12118763886083621No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGATGCTGGAACC60.12118763886083621No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA60.12118763886083621No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAGTGGCTAATGTAGAACCATGT60.12118763886083621No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCCTTGTTTTTAAGTGG50.10098969905069682No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT50.10098969905069682No Hit
AAATACCGTTCAGAGCTGCCCCCAGGAGCTTGCAAATTGCTGCTGGAACC50.10098969905069682No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA50.10098969905069682No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTTCAAATTGCTGCTGGAACC50.10098969905069682No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA50.10098969905069682No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTAATTCAATTAAATAC50.10098969905069682No Hit
AAATACCTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT50.10098969905069682No Hit
AAATACCGTTCAAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT50.10098969905069682No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA50.10098969905069682No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGATTGCAAATTGCTGCTGGAACC50.10098969905069682No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAA50.10098969905069682No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)