FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153007

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153007
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1662
Total Bases240.9 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTTGGACAGACCAAGTCTC56433.935018050541515No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG41524.969915764139593No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCT21312.815884476534295No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTTTGGACAGACCAAGTCTC1619.687123947051743No Hit
ACTTAAAAACAAGGCAGTGCTGAACGGTATTTTTGGACAGACCAAGTCTC311.865222623345367No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTGGACAGACCAAGTCTCT150.9025270758122743No Hit
ACTTAAAACAAGGCAGCTCTGAACGGTATTTTTGGACAGACCAAGTCTCT120.7220216606498195No Hit
ACTTAAAAACAAGGCAGCTCTGACGGTATTTTTGGACAGACCAAGTCTCT70.421179302045728No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTTGGACAGACCAAGTCTCT70.421179302045728No Hit
ACTTAAAAACAAGGCAGTGCTGTGCCCTCCCAGGTACCCCAAGCTGGCAG60.36101083032490977No Hit
TGTGTGTGTGTTTCTGTGGGTAAGCAGTGGTATCAACGCAGAGTACATGG60.36101083032490977Clontech Universal Primer Mix Long (96% over 27bp)
ACTTAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC50.3008423586040915No Hit
ACTTAAAAACAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC50.3008423586040915No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGATTTTTGGACAGACCAAGTCTCT50.3008423586040915No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTTGGACAGACAAAGTCTC50.3008423586040915No Hit
TGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGCT50.3008423586040915No Hit
ACTTAAAAACAAGGCAGCTCTGAACGTATTTTTGGACAGACCAAGTCTCT40.24067388688327318No Hit
ACTTAAAAACAAGGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGT40.24067388688327318Clontech SMARTer II A Oligonucleotide (96% over 25bp)
ACTTAAACAAGGCAGCTCTGAACGGTATTTTTGGACAGACCAAGTCTCTG40.24067388688327318No Hit
TTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC30.18050541516245489No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTTGGACAGCCCAAGTCTC30.18050541516245489No Hit
ACTTAAAAACAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA30.18050541516245489Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTTAAAAACAAGGCAGCTCTAACGGTATTTTTGGACAGACCAAGTCTCT30.18050541516245489No Hit
ACTTAAAAACAAGGCAGTGCTATCAACGAAGAGGTCCATTCTAGTGCCTT30.18050541516245489No Hit
ACTTAAAAACAAGGCAGTCTGAACGGTATTTTTGGACAGACCAAGTCTCT30.18050541516245489No Hit
ACTTAAAACAAGGCAGTTCTGAACGGTATTTTTGGACAGACCAAGTCTCT30.18050541516245489No Hit
ATTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC30.18050541516245489No Hit
ACTTAAAAACAAGGCACTCTGAACGGTATTTTTGGACAGACCAAGTCTCT30.18050541516245489No Hit
ACTTAAAAACAAGGCAGTGCTAACGCCTAATGGTGCTACAGTTTCTGCCT20.12033694344163659No Hit
ACTTAAAAACAAGGCGGCTCTGAACGGTATTTTTGGACAGACCAAGTCTC20.12033694344163659No Hit
ACTTAAAACAAGGCAGTGCTGAACGGTATTTTTGGACAGACCAAGTCTCT20.12033694344163659No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGAGTAAAG20.12033694344163659No Hit
ACTTAAAAACAAGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC20.12033694344163659No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGATTTTTGGACAGACCAAGTCTCT20.12033694344163659No Hit
ACTTAAAAACAAGGCAGTGGTATCAACGAAGAGGTCCATTCTAGTGCCTT20.12033694344163659No Hit
ACTTAAAAACAAGGCAGTTCTAATGCCTAATGGTGCTACAGTTTCTGCCT20.12033694344163659No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTTTTTTGGACAGACCAAGTCTCT20.12033694344163659No Hit
ACTTAAAAACAAGGCGGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCT20.12033694344163659No Hit
ACTTAAAAACAAGGCAGCTTGAACGGTATTTTTGGACAGACCAAGTCTCT20.12033694344163659No Hit
ACTTAAAAAAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC20.12033694344163659No Hit
ACTTAAAAACAAGGCAGTGCTATGCCCTCCCAGGTACCCCAAGCTGGCAG20.12033694344163659No Hit
TGTGTGTTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC20.12033694344163659No Hit
ACTTAAAAACAAGGCAGCTCTAAACGGTATTTTTGGACAGACCAAGTCTC20.12033694344163659No Hit
ACTTAAAAACAAGGCAGTTCTGAAGGTATTTTTGGACAGACCAAGTCTCT20.12033694344163659No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTTGTACAGACCAAGTCTC20.12033694344163659No Hit
ACTTAAAAACAAGGCAGCTCGAACGGTATTTTTGGACAGACCAAGTCTCT20.12033694344163659No Hit
TGTGTGTGTGTTTCTGTAGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG20.12033694344163659No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAGGGGTAAAG20.12033694344163659No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTCTAAGGTTTGGACAGAAGGGTAAAG20.12033694344163659No Hit
CTTAAAAACAAGGCAGCTCTGAACGGTATTTTTGGACAGACCAAGTCTCT20.12033694344163659No Hit
TGTGTGTGTGTTTCTGCGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG20.12033694344163659No Hit
TGTGTGTGTGTTTCTGTGGGTTCCTTTAAGGTTTGGACAGAAGGGTAAAG20.12033694344163659No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)