FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153008

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153008
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1662
Total Bases240.9 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC49

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG56033.69434416365824No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC24614.801444043321299No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC19411.672683513838749No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG1609.626955475330927No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC1106.618531889290012No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG301.8050541516245486No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT130.7821901323706378No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTGGCTAATGTAGAACCATGT120.7220216606498195No Hit
AATACCGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT80.48134777376654636No Hit
AAATACCGTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT70.421179302045728No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA70.421179302045728No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCTTCCATGTACTCTGC70.421179302045728Clontech SMARTer II A Oligonucleotide (95% over 22bp)
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT60.36101083032490977No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT60.36101083032490977No Hit
AAATCCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT50.3008423586040915No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGTTTGCAAATTGCTGCTGGAACC50.3008423586040915No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA40.24067388688327318No Hit
AAATACGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT40.24067388688327318No Hit
GGCACTAGAATGGACCTCTGCGTTGATACCACTGCTTCCATGTACTCTGC40.24067388688327318Clontech Universal Primer Mix Long (95% over 23bp)
AAATACCGTTCAGAGCTGCCTTGTTTAAGTGGCTAATGTAGAACCATGTC40.24067388688327318No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA30.18050541516245489No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTGG30.18050541516245489No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA30.18050541516245489No Hit
AAATACCGTTCAGAGTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT30.18050541516245489No Hit
AAATACCGTTCAGACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT30.18050541516245489No Hit
GGCACTAGAATGGACCTCTTCCCCATCACTACATTATTTCAATTAAATAC30.18050541516245489No Hit
AAATACCGTTCAGAACTGCCTTGTTTTAAGTGGCTAATGTAGAACCATGT30.18050541516245489No Hit
AAATACCGTTAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT30.18050541516245489No Hit
AAATCCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT20.12033694344163659No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCCTTGTTTTTAAGTGG20.12033694344163659No Hit
AAATACCGTTCGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT20.12033694344163659No Hit
AAATACCTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT20.12033694344163659No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA20.12033694344163659No Hit
AAATACCGTTCAGCACTGCCTTGTTTTAAGTGGCTAATGTAGAACCATGT20.12033694344163659No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA20.12033694344163659No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA20.12033694344163659No Hit
GGCACTAGAATGGACCCTTCCTCATCACTACATTATTTCAATTAAATACA20.12033694344163659No Hit
AAATACCTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT20.12033694344163659No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTAGAACC20.12033694344163659No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGGGCTAATGTAGAACCATGT20.12033694344163659No Hit
GGCACTAGAATGGCCTCTTCCTCATCACTACATTATTTCAATTAAATACA20.12033694344163659No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCTCCCATGTACTCTGC20.12033694344163659Clontech SMARTer II A Oligonucleotide (95% over 21bp)
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA20.12033694344163659No Hit
GGCACTAGAATGGACCTCTTCCTCATCGCTAAATTATTTCAATTAAATAC20.12033694344163659No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTAAATTAAATAC20.12033694344163659No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTACTTCAATTAAATAC20.12033694344163659No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA20.12033694344163659No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGTTGGAACC20.12033694344163659No Hit
GGCACTAGAATGGACCTTTCCTCATCACTACATTATTTCAATTAAATACA20.12033694344163659No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT20.12033694344163659No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA20.12033694344163659No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGACCC20.12033694344163659No Hit
AAATACCGTTCAGAGCTGCCACTAAGAGCTTGCAAATTGCTGCTGGAACC20.12033694344163659No Hit
GGCACTAGAATGGACCTCTTCGTCATCACTACATTATTTCAATTAAATAC20.12033694344163659No Hit
AAATACCGTTCAGAGCTGCCTTGTTTATAAGTGGCTAATGTAGAACCATG20.12033694344163659No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)