FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153016

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153016
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4334
Total Bases628.2 kbp
Sequences flagged as poor quality0
Sequence length141-145
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC98922.81956622058145No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG59513.728657129672358No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC57313.221042916474387No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC49611.444393170281495No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC48611.21365943700969No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG1924.430087678818643No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG360.8306414397784956No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT320.7383479464697739No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA210.4845408398707891No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT210.4845408398707891No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT170.3922473465620674No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA140.3230272265805261No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA140.3230272265805261No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA130.29995385325334567No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT130.29995385325334567No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA120.2768804799261652No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTGGCTAATGTAGAACCATGT120.2768804799261652No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT120.2768804799261652No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA110.25380710659898476No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTGG100.23073373327180433No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA100.23073373327180433No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTACATTATTTCAATTAAATAC80.18458698661744347No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA80.18458698661744347No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT70.16151361329026304No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA70.16151361329026304No Hit
AAATACCGTTCAGACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT70.16151361329026304No Hit
AAATACCTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT70.16151361329026304No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA70.16151361329026304No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA60.1384402399630826No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA60.1384402399630826No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGACCC60.1384402399630826No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA60.1384402399630826No Hit
GGCACTAGAATGGACCTCCTCCTCATCACTACATTATTTCAATTAAATAC60.1384402399630826No Hit
GGCACTAGAATGGACCTCTTCGTCATCACTAAATTATTTCAATTAAATAC50.11536686663590216No Hit
AAATCCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT50.11536686663590216No Hit
AAATACGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT50.11536686663590216No Hit
AAATACCGTTAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT50.11536686663590216No Hit
GGCACTAGAATGGACCCTTCCTCATCACTACATTATTTCAATTAAATACA50.11536686663590216No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA50.11536686663590216No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)